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Title:
GLYCOSYLATED HEMOGLOBIN ON-LINE HEMOLYTIC ENZYMATIC ASSAY KIT
Document Type and Number:
WIPO Patent Application WO/2018/233174
Kind Code:
A1
Abstract:
Disclosed is a glycosylated hemoglobin on-line hemolytic enzymatic assay kit, consisting of a reagent R1, a reagent R2 and a reagent R3. The reagent R1 comprises: 10-1,000 mmol/L of morpholinopropanesulfonic acid 3-(N-morpholine) propanesulfonic acid, 10-1,000 mmol/L of N-cyclohexyl-2-aminoethanesulfonic acid, 1-200 g/L of polyoxyethylene lauryl ether (lauryl polyoxyethylene ether), 100-10,000 kU/L of metalloprotease (in a reagent R1a), and 0.1-10 mmol/L of 2-(iodophenyl)-3-(2,4-dinitrobenzene)-5-(2,4-disulfophenyl)-2H-tetrazole sodium salt. The reagent R2 comprises 0.1-10 mmol/L of morpholinoethylsulfonic acid and 0.5-50 mmol/L of CaCl2. The reagent R3 comprises 10-1,000 mmol/L of Tris-HCl, 1-150 kU/L of high-activity fructose valine oxidase, 30-600 kU/L of peroxidase, and 0.01-0.5 mmol/L of N-(carboxymethylaminocarbonyl)-4,4-diazo(dimethylamine)-diphenylamine sodium salt.

Inventors:
ZOU BINGDE (CN)
ZOU JIHUA (CN)
JIA JIANGHUA (CN)
HE CAILANG (CN)
Application Number:
PCT/CN2017/108541
Publication Date:
December 27, 2018
Filing Date:
October 31, 2017
Export Citation:
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Assignee:
MEDICAL SYSTEM BIOTECHNOLOGY CO LTD (CN)
International Classes:
C12Q1/28; C12Q1/26; G01N21/78
Foreign References:
CN107164456A2017-09-15
CN101358229A2009-02-04
Attorney, Agent or Firm:
NINGBO WITS IP SERVICES CO., LTD (CN)
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