Title:
HUMAN SOLUBLE NEUROPILIN-1 PRIMARY POLYADENYLATION SIGNAL AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO2005073384
Kind Code:
A3
Abstract:
The human soluble neuropilin-1 (sNRP) polyadenylation signal (sNRP-poly(A)), situated downstream of the GT splice donor site of intron 12 of the full-length neuropilin-1 gene, also functions as the termination codon for sNRP. This 17 nucleotide sequence efficiently facilitates addition of poly(A) tails to RNAs expresses in cells. The present invention shows that this optimally succinct sequence has similar activity to the SV40 polyadenylation signal that is currently used in expression vectors. By using this shorter dual termination/polyadenylation signal and avoiding the need for large and cumbersome polyadenylation signals, expression vectors may be engineered to carry considerably larger genes.
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Inventors:
STOUT J TIMOTHY (US)
MCFARLAND TREVOR (US)
FRANCIS PETER J (US)
APPUKUTTAN BINOY
MCFARLAND TREVOR (US)
FRANCIS PETER J (US)
APPUKUTTAN BINOY
Application Number:
PCT/US2005/002620
Publication Date:
September 22, 2005
Filing Date:
January 28, 2005
Export Citation:
Assignee:
RES DEV FOUNDATION (US)
STOUT J TIMOTHY (US)
MCFARLAND TREVOR (US)
FRANCIS PETER J (US)
APPUKUTTAN BINOY
STOUT J TIMOTHY (US)
MCFARLAND TREVOR (US)
FRANCIS PETER J (US)
APPUKUTTAN BINOY
International Classes:
A61K48/00; C07H21/04; C07K14/71; C12N15/85; C12N15/86; (IPC1-7): C12N15/85
Domestic Patent References:
| WO1988002029A1 | 1988-03-24 |
Other References:
GAGNON MICHAEL L ET AL: "Identification of a natural soluble neuropilin-1 that binds vascular endothelial growth factor: In vivo expression and antitumor activity", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, vol. 97, no. 6, 14 March 2000 (2000-03-14), pages 2573 - 2578, XP002340211, ISSN: 0027-8424
COOKE CHARLES ET AL: "Characterization of specific protein-RNA complexes associated with the coupling of polyadenylation and last-intron removal", MOLECULAR AND CELLULAR BIOLOGY, vol. 22, no. 13, July 2002 (2002-07-01), pages 4579 - 4586, XP002340212, ISSN: 0270-7306
ZARUDNAYA MARGARITA I ET AL: "Downstream elements of mammalian pre-mRNA polyadenylation signals: Primary, secondary and higher-order structures.", NUCLEIC ACIDS RESEARCH, vol. 31, no. 5, 1 March 2003 (2003-03-01), pages 1375 - 1386, XP002340213, ISSN: 0305-1048
COLGAN D F ET AL: "MECHANISM AND REGULATION OF MRNA POLYADENYLATION", GENES AND DEVELOPMENT, COLD SPRING HARBOR LABORATORY PRESS, NEW YORK, US, vol. 11, no. 21, 1 November 1997 (1997-11-01), pages 2755 - 2766, XP001021773, ISSN: 0890-9369
CACKOWSKI F C ET AL: "Identification of two novel alternatively spliced Neuropilin-1 isoforms", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 84, no. 1, July 2004 (2004-07-01), pages 82 - 94, XP004519106, ISSN: 0888-7543
COOKE CHARLES ET AL: "Characterization of specific protein-RNA complexes associated with the coupling of polyadenylation and last-intron removal", MOLECULAR AND CELLULAR BIOLOGY, vol. 22, no. 13, July 2002 (2002-07-01), pages 4579 - 4586, XP002340212, ISSN: 0270-7306
ZARUDNAYA MARGARITA I ET AL: "Downstream elements of mammalian pre-mRNA polyadenylation signals: Primary, secondary and higher-order structures.", NUCLEIC ACIDS RESEARCH, vol. 31, no. 5, 1 March 2003 (2003-03-01), pages 1375 - 1386, XP002340213, ISSN: 0305-1048
COLGAN D F ET AL: "MECHANISM AND REGULATION OF MRNA POLYADENYLATION", GENES AND DEVELOPMENT, COLD SPRING HARBOR LABORATORY PRESS, NEW YORK, US, vol. 11, no. 21, 1 November 1997 (1997-11-01), pages 2755 - 2766, XP001021773, ISSN: 0890-9369
CACKOWSKI F C ET AL: "Identification of two novel alternatively spliced Neuropilin-1 isoforms", GENOMICS, ACADEMIC PRESS, SAN DIEGO, US, vol. 84, no. 1, July 2004 (2004-07-01), pages 82 - 94, XP004519106, ISSN: 0888-7543
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