IMPROVED β-FRUCTOFURANOSIDASE - FOOD SCIENCE CO LTD B

Title:

IMPROVED β-FRUCTOFURANOSIDASE

Document Type and Number:

WIPO Patent Application WO/2016/017774

Kind Code:

A1

Abstract:

[Problem] To provide an improved β-fructofuranosidase which can produce kestose while preventing the production of nystose and can decompose sucrose while preventing the decomposition of an oligosaccharide. [Solution] An improved β-fructofuranosidase which comprises an amino acid sequence (a) or (b): (a) an amino acid sequence which is produced by introducing an amino acid mutation into an amino acid sequence for a wild-type β-fructofuranosidase which is represented by SEQ ID NO: 2, wherein the amino acid mutation can cause the substitution of a histidine residue (H) located at position-321 as counted from the N-terminal of the amino acid sequence for the wild-type β-fructofuranosidase by a protein-constituting amino acid residue other than a histidine residue (H); and (b) an amino acid sequence which is produced by deleting, substituting, inserting or adding one or several amino acid residues, each of which is different from the amino acid residue into which the amino acid mutation has been introduced, in the amino acid sequence (a), and which has a β-fructofuranosidase activity.

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Inventors:

TOCHIO TAKUMI (JP)
ITO YUMI (JP)
FUJII TADASHI (JP)
TAMURA KEISUKE (JP)

Application Number:

PCT/JP2015/071701

Publication Date:

February 04, 2016

Filing Date:

July 30, 2015

Export Citation:

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Assignee:

FOOD SCIENCE CO LTD B (JP)
MICROBIOPHARM JAPAN CO LTD (JP)

International Classes:

C12N9/24; C12N1/21; C12N15/09

Domestic Patent References:

WO2005085447A1

2005-09-15

Foreign References:

CN1772903A	2006-05-17
JP2010273580A	2010-12-09
JP3628336B2	2005-03-09

Other References:

JAKOB F. ET AL.: "Comparison of novel GH 68 levansucrases of levan-overproducing Gluconobacter species", ACETIC ACID BACTERIA, vol. 1, no. e2, 2012, pages 6 - 14

Attorney, Agent or Firm:

SAGAWA Shingo et al. (JP)
Shingo Sagawa (JP)

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