Title:
INSULIN SECRETION PROMOTING PEPTIDE
Document Type and Number:
WIPO Patent Application WO/2020/158491
Kind Code:
A1
Abstract:
The present invention provides a peptide that promotes insulin secretion, and a use for said peptide. A search for insulin secretion promoting factors present in a culture supernatant of brown adipocytes (BA-SUP) derived from human pluripotent stem cells led to the identification of a bioactive peptide that promotes insulin secretion. Through the development of a peptide that imitates said peptide, it was discovered that a short-chain linear peptide capable of forming an α-helix structure exhibits strong insulin secretion promoting activity. Moreover, it was discovered that by heat-treating said peptide under acidic conditions or freeze-drying the peptide after dissolving in a buffer solution, the peptide becomes an active form having an increased insulin secretion promoting effect. Said peptide exhibited an effect of promoting insulin secretion using pancreatic beta cells, and of reducing the blood sugar level in vivo. The peptide group according to the present invention is expected to be applicable to the development of a therapeutic medication for glucose metabolism disorders.
Inventors:
SAEKI KUMIKO (JP)
OKA MASAKO (JP)
MATSUMURA KAZUNORI (JP)
SHU TSUGUMINE (JP)
MORI TOYOTAKA (JP)
OKA MASAKO (JP)
MATSUMURA KAZUNORI (JP)
SHU TSUGUMINE (JP)
MORI TOYOTAKA (JP)
Application Number:
PCT/JP2020/001791
Publication Date:
August 06, 2020
Filing Date:
January 21, 2020
Export Citation:
Assignee:
NAT CENTER FOR GLOBAL HEALTH AND MEDICINE (JP)
ID PHARMA CO LTD (JP)
ID PHARMA CO LTD (JP)
International Classes:
A61P3/08; A61K38/08; A61K38/10; A61K38/12; A61K47/50; A61K47/65; A61P3/10; A61P43/00; C07K7/06; C07K7/64; C12N15/11
Domestic Patent References:
| WO2018209127A1 | 2018-11-15 | |||
| WO2017223528A1 | 2017-12-28 | |||
| WO2020036184A1 | 2020-02-20 |
Foreign References:
| US20040214272A1 | 2004-10-28 |
Other References:
DIKEAKOS, J. D. ET AL.: "A Hydrophobic Patch in a Charged alfa-Helix Is Sufficient to Target Proteins to Dense Core Secretory Granules", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 282, no. 2, 12 January 2007 (2007-01-12), pages 1136 - 1143, XP055726288
ODDO, A. ET AL.: "a-Helix or beta-Turn? An Investigation into N-Terminally Constrained Analogues of Glucagon-like Peptide 1 (GLP-1) and Exendin-4", BIOCHEMISTRY, vol. 57, 7 June 2018 (2018-06-07), pages 4148 - 4154, XP055726291
PERU TZ, M. F. ET AL.: "Aggregation of proteins with expanded glutamine and alanine repeats of the glutamine-rich and asparagine-rich domains of Sup35 and of the amyloid beta-peptide of amyloid plaques", PNAS, vol. 99, no. 8, 16 April 2002 (2002-04-16), pages 5596 - 5600, XP055726293
DATABASE UniProtKB 5 February 2008 (2008-02-05), "SubName: Full=Predicted protein {ECO:0000313|EMBL:EDQ59095.1}", XP055726296, Database accession no. A9TBS2
DATABASE UniProtKB 7 November 2018 (2018-11-07), "SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:RBP35336.1}", XP055726297, Database accession no. AOA366H3G4
ODDO, A. ET AL.: "a-Helix or beta-Turn? An Investigation into N-Terminally Constrained Analogues of Glucagon-like Peptide 1 (GLP-1) and Exendin-4", BIOCHEMISTRY, vol. 57, 7 June 2018 (2018-06-07), pages 4148 - 4154, XP055726291
PERU TZ, M. F. ET AL.: "Aggregation of proteins with expanded glutamine and alanine repeats of the glutamine-rich and asparagine-rich domains of Sup35 and of the amyloid beta-peptide of amyloid plaques", PNAS, vol. 99, no. 8, 16 April 2002 (2002-04-16), pages 5596 - 5600, XP055726293
DATABASE UniProtKB 5 February 2008 (2008-02-05), "SubName: Full=Predicted protein {ECO:0000313|EMBL:EDQ59095.1}", XP055726296, Database accession no. A9TBS2
DATABASE UniProtKB 7 November 2018 (2018-11-07), "SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:RBP35336.1}", XP055726297, Database accession no. AOA366H3G4
Attorney, Agent or Firm:
HARUNA, Masao et al. (JP)
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