Title:
METHOD FOR PRODUCING HIGH-QUALITY iPS CELLS
Document Type and Number:
WIPO Patent Application WO/2017/010080
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide an iPS cell quality improver, a method for producing iPS cells, iPS cells produced using said production method, and a composition for producing iPS cells. This method for producing iPS cells is characterized by containing a step in which (a) nuclear reprogramming substance and (b) H1foo gene or gene product thereof are introduced into somatic cells. By introducing not only a nuclear reprogramming substance but also a H1foo gene or a gene product thereof into somatic cells, it is possible to produce high-quality iPS cells in greater quantity.
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Inventors:
YUASA SHINSUKE (JP)
FUKUDA KEIICHI (JP)
KUNITOMI AKIRA (JP)
FUKUDA KEIICHI (JP)
KUNITOMI AKIRA (JP)
Application Number:
PCT/JP2016/003282
Publication Date:
January 19, 2017
Filing Date:
July 11, 2016
Export Citation:
Assignee:
HEARTSEED INC (JP)
International Classes:
C12N5/10; C12N15/09; C07K14/47
Domestic Patent References:
| WO2009114949A1 | 2009-09-24 |
Other References:
KUNITOMI A. ET AL.: "Hlfoo has a pivotal role in qualifying induced pluripotent stem cells.", STEM CELL REPORTS, vol. 6, 26 May 2016 (2016-05-26), pages 825 - 833, XP055509242
AWE J.P . ET AL.: "Identifying candidate oocyte reprogramming factors using cross- species global transcriptional analysis.", CELLULAR REPROGRAMMING, vol. 15, no. 2, 2013, pages 126 - 133, XP002747890, DOI: doi:10.1089/cell.2012.0060
MAEKAWA M. ET AL.: "Direct reprogramming of somatic cells is promoted by maternal transcription factor Glisl.", NATURE, vol. 474, 2011, pages 225 - 229, XP055034083, DOI: doi:10.1038/nature10106
SHINAGAWA T. ET AL.: "Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells.", CELL STEM CELL, vol. 14, 2014, pages 217 - 227, XP002747891, DOI: doi:10.1016/j.stem.2013.12.015
BRINK T.C. ET AL.: "The origins of human embryonic stem cells: A biological conundrum.", CELLS TISSUES ORGANS, vol. 188, 2008, pages 9 - 22, XP009508280
HAYAKAWA K. ET AL.: "Oocyte-specific linker histone Hlhoo is an epigenomic modulator that decondenses chromatin and impairs pluripotency.", EPIGENETICS, vol. 7, no. 9, 2012, pages 1029 - 1036, XP055509257
NASHUN B. ET AL.: "Reprogramming of cell fate: epigenetic memory and the erasure of memories past.", THE EMBO JOURNAL, vol. 34, 27 March 2015 (2015-03-27), pages 1296 - 1308, XP055509261
See also references of EP 3333256A4
AWE J.P . ET AL.: "Identifying candidate oocyte reprogramming factors using cross- species global transcriptional analysis.", CELLULAR REPROGRAMMING, vol. 15, no. 2, 2013, pages 126 - 133, XP002747890, DOI: doi:10.1089/cell.2012.0060
MAEKAWA M. ET AL.: "Direct reprogramming of somatic cells is promoted by maternal transcription factor Glisl.", NATURE, vol. 474, 2011, pages 225 - 229, XP055034083, DOI: doi:10.1038/nature10106
SHINAGAWA T. ET AL.: "Histone variants enriched in oocytes enhance reprogramming to induced pluripotent stem cells.", CELL STEM CELL, vol. 14, 2014, pages 217 - 227, XP002747891, DOI: doi:10.1016/j.stem.2013.12.015
BRINK T.C. ET AL.: "The origins of human embryonic stem cells: A biological conundrum.", CELLS TISSUES ORGANS, vol. 188, 2008, pages 9 - 22, XP009508280
HAYAKAWA K. ET AL.: "Oocyte-specific linker histone Hlhoo is an epigenomic modulator that decondenses chromatin and impairs pluripotency.", EPIGENETICS, vol. 7, no. 9, 2012, pages 1029 - 1036, XP055509257
NASHUN B. ET AL.: "Reprogramming of cell fate: epigenetic memory and the erasure of memories past.", THE EMBO JOURNAL, vol. 34, 27 March 2015 (2015-03-27), pages 1296 - 1308, XP055509261
See also references of EP 3333256A4
Attorney, Agent or Firm:
HIROTA, Masanori (JP)
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