Title:
A METHOD OF PRODUCING IgG
Document Type and Number:
WIPO Patent Application WO2001072844
Kind Code:
A3
Abstract:
A method for producing IgG from plasma for medical applications, comprising at least: (i') removal of albumin resulting in an IgG fraction, (ii') purifying IgG from an IgG fraction, which is derived from the IgG fraction obtained in step (i'), by adsorbing IgG to a cation exchanger and collecting the adsorbed IgG fraction, and (iii') virus inactivation in an IgG fraction derived from the IgG fraction collected in step (ii'). The method is characterized in; (I) concentrating the IgG fraction obtained in step (i'), (II) adjusting pH to 4+/-0.1 in the IgG fraction released from the cation exchanger in step (ii'), and preferably maintaining the pH below 6.0 during the remaining steps of the method; and (III) carrying out the virus inactivation (step iii') by using chemicals at a temperature of 30 DEG C +/- 2 DEG C for at least 4 hours. Anticomplementary activity is typically below 1 CH50/mg immunoglobulin.
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Inventors:
ANDERSSON INGER (SE)
LINDQUIST LARS-OLOF (SE)
LINDQUIST LARS-OLOF (SE)
Application Number:
PCT/EP2001/003624
Publication Date:
April 11, 2002
Filing Date:
March 29, 2001
Export Citation:
Assignee:
AMERSHAM BIOSCIENCES AB (SE)
ANDERSSON INGER (SE)
LINDQUIST LARS OLOF (SE)
ANDERSSON INGER (SE)
LINDQUIST LARS OLOF (SE)
International Classes:
C07K1/18; C07K1/34; C07K16/06; (IPC1-7): C07K16/06; C07K1/18; A61K39/395
Domestic Patent References:
| WO1999064462A1 | 1999-12-16 | |||
| WO1999018130A1 | 1999-04-15 |
Foreign References:
| US5593675A | 1997-01-14 | |||
| DE4118912C1 | 1992-07-02 | |||
| US5410025A | 1995-04-25 | |||
| US5164487A | 1992-11-17 |
Other References:
CHRISTINA ÖSTLUND: "Large-scale purification of monoclonal antibodies", TIBTECH, November 1986 (1986-11-01), pages 288 - 293, XP002902147
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