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Patent Searching and Data


Title:
METHOD FOR ACQUIRING BASE SEQUENCE INFORMATION ABOUT SINGLE CELLS DERIVED FROM VERTEBRATE ANIMALS
Document Type and Number:
WIPO Patent Application WO/2018/061674
Kind Code:
A1
Abstract:
Provided is a method for acquiring base sequence information about single cells derived from vertebrate animals, said method permitting uniform, accurate PCR amplification of a target region.

Inventors:
TSUJIMOTO TAKAYUKI (JP)
ISHII YASUYUKI (JP)
INOUE YUKI (JP)
OUCHI AYA (JP)
NAKATANI TOSHIYUKI (JP)
ENDOH SETSU (JP)
Application Number:
PCT/JP2017/032110
Publication Date:
April 05, 2018
Filing Date:
September 06, 2017
Export Citation:
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Assignee:
FUJIFILM CORP (JP)
International Classes:
C12Q1/68; C12Q1/02; C12N15/09
Domestic Patent References:
WO2016042830A12016-03-24
WO2004081225A22004-09-23
WO2008004691A12008-01-10
WO2012023298A12012-02-23
Foreign References:
JP2011163830A2011-08-25
JP2013042689A2013-03-04
Other References:
NOVAK, R. ET AL.: "Single- Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose Emulsions", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 50, no. 2, 2011, pages 390 - 395, XP002629259, DOI: doi:10.1002/ANIE.201006089
PONTEN, F. ET AL.: "Genomic analysis of single cells from human basal cell cancer using laser -assisted capture microscopy", MUTATION RESEARCH GENOMICS, vol. 382, 1997, pages 45 - 55, XP055585551
KAPLINSKI, L. ET AL.: "MultiPLX: Automatic grouping and evaluation of PCR primers", PCR PRIMER DESIGN, vol. 21, no. 8, 15 April 2005 (2005-04-15), pages 1701 - 1702, XP055227877
LI, H. ET AL.: "Fast and accurate short read alignment with Burrows-Wheeler transform", BIOINFORMATICS, vol. 25, no. 14, 2009, pages 1754 - 1760, XP055287430, DOI: doi:10.1093/bioinformatics/btp324
LI, H. ET AL.: "Fast and accurate long-read alignment with Burrows-Wheeler transform", BIOINFORMATICS, vol. 26, no. 5, 2010, pages 589 - 595
LI, HENG ET AL.: "The Sequence Alignment / Map format and SAMtools", BIOINFORMATICS, vol. 25, no. 16, 2009, pages 2078 - 2079, XP055229864, DOI: doi:10.1093/bioinformatics/btp352
QUINLAN, A. R. ET AL.: "BEDTools: a flexible suite of utilities for comparing genomic features", BIOINFORMATICS, vol. 26, no. 6, 2010, pages 841 - 842, XP055307411, DOI: doi:10.1093/bioinformatics/btq033
ALTSCHUL, S. A. ET AL.: "Basic Local Alignment Search Tool", JOURNAL OF MOLECULAR BIOLOGY, vol. 215, October 1990 (1990-10-01), pages 403 - 410, XP002949123, DOI: doi:10.1006/jmbi.1990.9999
PEARSON, W. R. ET AL.: "Proceedings of the National Academy of Sciences of the United States of America", vol. 85, April 1988, NATIONAL ACADEMY OF SCIENCES, article "Improved tools for biological sequence comparison", pages: 2444 - 2448
Attorney, Agent or Firm:
WATANABE Mochitoshi et al. (JP)
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