Title:
METHOD FOR ACQUIRING BASE SEQUENCE INFORMATION ABOUT SINGLE CELLS DERIVED FROM VERTEBRATE ANIMALS
Document Type and Number:
WIPO Patent Application WO/2018/061674
Kind Code:
A1
Abstract:
Provided is a method for acquiring base sequence information about single cells derived from vertebrate animals, said method permitting uniform, accurate PCR amplification of a target region.
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Inventors:
TSUJIMOTO TAKAYUKI (JP)
ISHII YASUYUKI (JP)
INOUE YUKI (JP)
OUCHI AYA (JP)
NAKATANI TOSHIYUKI (JP)
ENDOH SETSU (JP)
ISHII YASUYUKI (JP)
INOUE YUKI (JP)
OUCHI AYA (JP)
NAKATANI TOSHIYUKI (JP)
ENDOH SETSU (JP)
Application Number:
PCT/JP2017/032110
Publication Date:
April 05, 2018
Filing Date:
September 06, 2017
Export Citation:
Assignee:
FUJIFILM CORP (JP)
International Classes:
C12Q1/68; C12Q1/02; C12N15/09
Domestic Patent References:
WO2016042830A1 | 2016-03-24 | |||
WO2004081225A2 | 2004-09-23 | |||
WO2008004691A1 | 2008-01-10 | |||
WO2012023298A1 | 2012-02-23 |
Foreign References:
JP2011163830A | 2011-08-25 | |||
JP2013042689A | 2013-03-04 |
Other References:
NOVAK, R. ET AL.: "Single- Cell Multiplex Gene Detection and Sequencing with Microfluidically Generated Agarose Emulsions", ANGEWANDTE CHEMIE INTERNATIONAL EDITION, vol. 50, no. 2, 2011, pages 390 - 395, XP002629259, DOI: doi:10.1002/ANIE.201006089
PONTEN, F. ET AL.: "Genomic analysis of single cells from human basal cell cancer using laser -assisted capture microscopy", MUTATION RESEARCH GENOMICS, vol. 382, 1997, pages 45 - 55, XP055585551
KAPLINSKI, L. ET AL.: "MultiPLX: Automatic grouping and evaluation of PCR primers", PCR PRIMER DESIGN, vol. 21, no. 8, 15 April 2005 (2005-04-15), pages 1701 - 1702, XP055227877
LI, H. ET AL.: "Fast and accurate short read alignment with Burrows-Wheeler transform", BIOINFORMATICS, vol. 25, no. 14, 2009, pages 1754 - 1760, XP055287430, DOI: doi:10.1093/bioinformatics/btp324
LI, H. ET AL.: "Fast and accurate long-read alignment with Burrows-Wheeler transform", BIOINFORMATICS, vol. 26, no. 5, 2010, pages 589 - 595
LI, HENG ET AL.: "The Sequence Alignment / Map format and SAMtools", BIOINFORMATICS, vol. 25, no. 16, 2009, pages 2078 - 2079, XP055229864, DOI: doi:10.1093/bioinformatics/btp352
QUINLAN, A. R. ET AL.: "BEDTools: a flexible suite of utilities for comparing genomic features", BIOINFORMATICS, vol. 26, no. 6, 2010, pages 841 - 842, XP055307411, DOI: doi:10.1093/bioinformatics/btq033
ALTSCHUL, S. A. ET AL.: "Basic Local Alignment Search Tool", JOURNAL OF MOLECULAR BIOLOGY, vol. 215, October 1990 (1990-10-01), pages 403 - 410, XP002949123, DOI: doi:10.1006/jmbi.1990.9999
PEARSON, W. R. ET AL.: "Proceedings of the National Academy of Sciences of the United States of America", vol. 85, April 1988, NATIONAL ACADEMY OF SCIENCES, article "Improved tools for biological sequence comparison", pages: 2444 - 2448
PONTEN, F. ET AL.: "Genomic analysis of single cells from human basal cell cancer using laser -assisted capture microscopy", MUTATION RESEARCH GENOMICS, vol. 382, 1997, pages 45 - 55, XP055585551
KAPLINSKI, L. ET AL.: "MultiPLX: Automatic grouping and evaluation of PCR primers", PCR PRIMER DESIGN, vol. 21, no. 8, 15 April 2005 (2005-04-15), pages 1701 - 1702, XP055227877
LI, H. ET AL.: "Fast and accurate short read alignment with Burrows-Wheeler transform", BIOINFORMATICS, vol. 25, no. 14, 2009, pages 1754 - 1760, XP055287430, DOI: doi:10.1093/bioinformatics/btp324
LI, H. ET AL.: "Fast and accurate long-read alignment with Burrows-Wheeler transform", BIOINFORMATICS, vol. 26, no. 5, 2010, pages 589 - 595
LI, HENG ET AL.: "The Sequence Alignment / Map format and SAMtools", BIOINFORMATICS, vol. 25, no. 16, 2009, pages 2078 - 2079, XP055229864, DOI: doi:10.1093/bioinformatics/btp352
QUINLAN, A. R. ET AL.: "BEDTools: a flexible suite of utilities for comparing genomic features", BIOINFORMATICS, vol. 26, no. 6, 2010, pages 841 - 842, XP055307411, DOI: doi:10.1093/bioinformatics/btq033
ALTSCHUL, S. A. ET AL.: "Basic Local Alignment Search Tool", JOURNAL OF MOLECULAR BIOLOGY, vol. 215, October 1990 (1990-10-01), pages 403 - 410, XP002949123, DOI: doi:10.1006/jmbi.1990.9999
PEARSON, W. R. ET AL.: "Proceedings of the National Academy of Sciences of the United States of America", vol. 85, April 1988, NATIONAL ACADEMY OF SCIENCES, article "Improved tools for biological sequence comparison", pages: 2444 - 2448
Attorney, Agent or Firm:
WATANABE Mochitoshi et al. (JP)
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