Title:
METHOD FOR CONSTRUCTING A SEQUENCING LIBRARY BASED ON A SINGLE-STRANDED DNA MOLECULE AND APPLICATION THEREOF
Document Type and Number:
WIPO Patent Application WO/2016/037537
Kind Code:
A1
Abstract:
A method for constructing a sequencing library based on a single-stranded DNA molecule is provided comprising: (1) forming a poly (C) n tail at a 3'-terminus of the single-stranded DNA molecule, to obtain a single-stranded DNA molecule with the poly (C) n tail with n representing a number of base C, and n being an integer ranging from 5 to 30; (2) obtaining a double-stranded DNA molecule by using an extension primer based on the single-stranded DNA molecule with the poly (C) n tail, with the extension primer comprising a H (G) m unit at a 3'-terminus thereof, H being base A, base T or base C, m being a number of base G, and m being an integer ranging from 5 to 15; and (3) ligating an adapter to one terminus of the double-stranded DNA molecule remote from the H (G) m unit, and amplifying the resulting ligation product to obtain an amplification product forming the sequencing library.
Inventors:
XIE WEI (CN)
YIN QIANGZONG (CN)
WU JINGYI (CN)
XU FENG (CN)
PENG XU (CN)
YIN QIANGZONG (CN)
WU JINGYI (CN)
XU FENG (CN)
PENG XU (CN)
Application Number:
PCT/CN2015/088680
Publication Date:
March 17, 2016
Filing Date:
August 31, 2015
Export Citation:
Assignee:
UNIV TSINGHUA (CN)
SINGAPORE INST FOR CLINICAL SCIENCES A STAR (SG)
SINGAPORE INST FOR CLINICAL SCIENCES A STAR (SG)
International Classes:
C12N15/10; C40B50/06; C12Q1/68; C40B40/06
Domestic Patent References:
| WO2004081225A2 | 2004-09-23 |
Attorney, Agent or Firm:
TSINGYIHUA INTELLECTUAL PROPERTY LLC (Trade Building Zhaolanyuan, Tsinghua University, Qinghuayuan, Haidian District, Beijing 4, CN)
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