Title:
METHOD FOR DETECTING NUCLEIC ACID
Document Type and Number:
WIPO Patent Application WO/2012/108499
Kind Code:
A1
Abstract:
The present invention provides: a method for detecting a nucleic acid, which efficiently eliminates non-specific detection of nucleic acids other than the nucleic acid to be detected, so that detection specificity of the nucleic acid to be detected can be further improved; or the like. This method for detecting a nucleic acid comprises: (a) a step wherein a gel, on which a probe is immobilized, is brought into contact with a reaction solution which contains a nucleic acid that serves as a template for nucleic acid amplification, a primer set for nucleic acid amplification, a nucleotide unit and a DNA extension enzyme; (b) a step wherein the gel and the reaction solution are subjected to a heat cycle for performing a nucleic acid amplification reaction; (c) a step wherein nucleic acid fragments having a specific base length are selected from among the amplified nucleic acid fragments; and (d) a step wherein the selected nucleic acid fragments are detected.
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Inventors:
TOGAWA NAOYUKI (JP)
Application Number:
PCT/JP2012/052968
Publication Date:
August 16, 2012
Filing Date:
February 09, 2012
Export Citation:
Assignee:
MITSUBISHI RAYON CO (JP)
TOGAWA NAOYUKI (JP)
TOGAWA NAOYUKI (JP)
International Classes:
C12N15/09; C12M1/00; C12Q1/68; G01N37/00
Domestic Patent References:
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| WO2000053736A1 | 2000-09-14 |
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Other References:
KOLCHINSKY A. ET AL.: "Analysis of SNPs and other genomic variations using gel-based chips.", HUM. MUTAT., vol. 19, no. 4, 2002, pages 343 - 360, XP008164154
ADESSI, CELINE ET AL.: "Solid phase DNA amplification: Characterisation of primer attachment and amplification mechanisms", NUCLEIC ACIDS RES., vol. 20, no. 20, 2000, pages E87, XP002456871, DOI: doi:10.1093/nar/28.20.e87
NUCLEIC ACIDS RES., January 2004 (2004-01-01)
GEORG, M. ET AL.: "Microarray-Based Identification of Bacteria in Clinical Samples by Solid-Phase PCR Amplification of 23S Ribosomal DNA Sequences", JOURNAL OF CLINICAL MICROBIOLOGY, March 2004 (2004-03-01), pages 1048 - 1057, XP002363929, DOI: doi:10.1128/JCM.42.3.1048-1057.2004
MARTIN H. ET AL.: "Detection of Single Base Alterations in Genomic DNA by Solid Phase Polymerase Chain Reaction on Oligonucleotide Microarrays", ANALYTICAL BIOCHEMISTRY, vol. 299, 2001, pages 24 - 30, XP002197619
SVETLANA D. ET AL.: "Polymorphism analysis and gene detection by minisequencing on an array of gel-immobilized primers", NUCLEIC ACIDES RES., vol. 27, no. 18, 1999, pages EL9
GENNADY YERSHOV ET AL.: "DNA analysis and diagnostics on oligonucleotide microchips", PROC. NATL. ACAD. SCI. USA, vol. 93, May 1996 (1996-05-01), pages 4913 - 4918
A. YU; RUBINA ET AL.: "Hydrogel drop microchips with immobilized DNA: properties and methods for large-scale production", ANALYTICAL BIOCHEMISTRY, vol. 325, 2004, pages 92 - 106, XP004483538, DOI: doi:10.1016/j.ab.2003.10.010
DMITRY A. KHODAKOV ET AL.: "An oligonucleotide microarray for multiplex realtime PCR identification ofHIV-1, HBV, and HCV", BIOTECHNIQUES, vol. 44, no. 2, 2008, pages 241 - 248
SHUJI SAKOHARA ET AL.: "Application of thermosensitive porous gel for construction of DNA chips", THE 69TH ANNUAL MEETING OF SOCIETY OF CHEMICAL ENGINEERS, pages 1316
BLIN ET AL., NUCLEIC ACIDS RES., vol. 3, 1976, pages 2303
FAVALORO ET AL., METHODS. ENZYMOL., vol. 65, 1980, pages 718
GENNADY YERSHOV ET AL., PROC. NATL. ACAD. SCI. USA, vol. 93, May 1996 (1996-05-01), pages 4913 - 4918
A. YU. RUBINA ET AL., ANALYTICAL BIOCHEMISTRY, vol. 325, 2004, pages 92 - 106
See also references of EP 2674492A4
ADESSI, CELINE ET AL.: "Solid phase DNA amplification: Characterisation of primer attachment and amplification mechanisms", NUCLEIC ACIDS RES., vol. 20, no. 20, 2000, pages E87, XP002456871, DOI: doi:10.1093/nar/28.20.e87
NUCLEIC ACIDS RES., January 2004 (2004-01-01)
GEORG, M. ET AL.: "Microarray-Based Identification of Bacteria in Clinical Samples by Solid-Phase PCR Amplification of 23S Ribosomal DNA Sequences", JOURNAL OF CLINICAL MICROBIOLOGY, March 2004 (2004-03-01), pages 1048 - 1057, XP002363929, DOI: doi:10.1128/JCM.42.3.1048-1057.2004
MARTIN H. ET AL.: "Detection of Single Base Alterations in Genomic DNA by Solid Phase Polymerase Chain Reaction on Oligonucleotide Microarrays", ANALYTICAL BIOCHEMISTRY, vol. 299, 2001, pages 24 - 30, XP002197619
SVETLANA D. ET AL.: "Polymorphism analysis and gene detection by minisequencing on an array of gel-immobilized primers", NUCLEIC ACIDES RES., vol. 27, no. 18, 1999, pages EL9
GENNADY YERSHOV ET AL.: "DNA analysis and diagnostics on oligonucleotide microchips", PROC. NATL. ACAD. SCI. USA, vol. 93, May 1996 (1996-05-01), pages 4913 - 4918
A. YU; RUBINA ET AL.: "Hydrogel drop microchips with immobilized DNA: properties and methods for large-scale production", ANALYTICAL BIOCHEMISTRY, vol. 325, 2004, pages 92 - 106, XP004483538, DOI: doi:10.1016/j.ab.2003.10.010
DMITRY A. KHODAKOV ET AL.: "An oligonucleotide microarray for multiplex realtime PCR identification ofHIV-1, HBV, and HCV", BIOTECHNIQUES, vol. 44, no. 2, 2008, pages 241 - 248
SHUJI SAKOHARA ET AL.: "Application of thermosensitive porous gel for construction of DNA chips", THE 69TH ANNUAL MEETING OF SOCIETY OF CHEMICAL ENGINEERS, pages 1316
BLIN ET AL., NUCLEIC ACIDS RES., vol. 3, 1976, pages 2303
FAVALORO ET AL., METHODS. ENZYMOL., vol. 65, 1980, pages 718
GENNADY YERSHOV ET AL., PROC. NATL. ACAD. SCI. USA, vol. 93, May 1996 (1996-05-01), pages 4913 - 4918
A. YU. RUBINA ET AL., ANALYTICAL BIOCHEMISTRY, vol. 325, 2004, pages 92 - 106
See also references of EP 2674492A4
Attorney, Agent or Firm:
KOBAYASHI Hiroshi et al. (JP)
Kobayashi 浩 (JP)
Kobayashi 浩 (JP)
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