Title:
METHOD FOR DISPLAYING BISPECIFIC ANTIBODY ON SURFACE OF MAMMALIAN CELL AND VECTOR
Document Type and Number:
WIPO Patent Application WO/2021/190631
Kind Code:
A1
Abstract:
Provided are a method for constructing a bispecific antigen binding polypeptide expression vector and the bispecific antigen binding polypeptide expression vector produced according to the method. The method comprises: performing treatment by using restriction endonuclease of a specific recognition enzyme cutting site to obtain seven nucleic acid fragments having specific cohesive ends; and directionally ligating the nucleic acid fragments. Further provided is a method for establishing a polypeptide display library by using the expression vector. The display library can be used for effectively screening antibodies or antibody fragments.
Inventors:
ZHOU CHEN (CN)
Application Number:
PCT/CN2021/083248
Publication Date:
September 30, 2021
Filing Date:
March 26, 2021
Export Citation:
Assignee:
DDBIO CO LTD SHANG HAI (CN)
International Classes:
A61K39/395; C07K16/18
Domestic Patent References:
| WO2019222428A1 | 2019-11-21 |
Foreign References:
| CN101210241A | 2008-07-02 | |||
| CN102978713A | 2013-03-20 | |||
| CN110691796A | 2020-01-14 |
Other References:
IVAN ZHOU ET AL.: "Four-way ligation for construction of a mammalian cell-based full-length antibody display library", ACTA BIOCHIM BIOPHYS SIN, vol. 43, 2011, pages 232 - 238
See also references of EP 4129332A4
See also references of EP 4129332A4
Attorney, Agent or Firm:
SHANGHAI DIANSHI PARTNERS, P.C. (CN)
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