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Title:
METHOD FOR EXTRACTING AND AMPLIFYING NUCLEIC ACID USING MAGNETIC PARTICLES
Document Type and Number:
WIPO Patent Application WO/2017/051939
Kind Code:
A1
Abstract:
The present invention, with respect to a method for extracting and amplifying nucleic acid which repeats, one or more times, a DNA PCR process comprising the steps of denaturing DNA, attaching a primer, and extending the DNA chain, relates to a method for extracting and amplifying nucleic acid characterized by using the resulting modified DNA-magnetic particle precipitate as is without eluting the modified DNA/RNA from the magnetic particles, and executing the primer attachment step and DNA chain extension step after washing the modified DNA-magnetic particle precipitate by means of a protein-containing washing solution. According to the present invention, first, highly sensitive and effective PCR can be carried out even with a sample having a low DNA content as the DNA damage in the sample can be prevented, second, highly sensitive and effective PCR of DNA can be carried out even under the presence of a solid support as the surface of same is nonspecifically blocked with a protein, and third, DNA contamination can be prevented as the DNA denaturation step and PCR step can be carried out in the same container.

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Inventors:
KIM TAI SUN (KR)
SONG KEUM SOO (KR)
Application Number:
PCT/KR2015/010008
Publication Date:
March 30, 2017
Filing Date:
September 23, 2015
Export Citation:
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Assignee:
BIOMETRIX TECH INC (KR)
International Classes:
C12N15/10; C12Q1/68
Foreign References:
JP2011229488A2011-11-17
KR100762969B12007-10-04
KR100601972B12006-07-18
KR20150127917A2015-11-18
Other References:
BAI, YALONG ET AL.: "A Rapid Method for the Detection of Foodborne Pathogens by Extraction of a Trace Amount of DNA from Raw Milk Based on Amino-Modified Silica-Coated Magnetic Nanoparticles and Polymerase Chain Reaction", ANALYTIC, CHIMICA, ACTA., vol. 787, no. 2014, 2 June 2013 (2013-06-02), pages 93 - 101, XP028576856
Attorney, Agent or Firm:
KOREANA PATENT FIRM (KR)
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