METHOD FOR NUCLEIC ACID AMPLIFICATION

WIPO Patent Application WO/2016/052619

A1

The purpose of the present invention is to provide a method for amplification of nucleic acid using RNA as the template, in which the method is capable of the following: eliminating the risk of nonspecific amplification caused by DNA contamination from the reagent and work environment; elevating trace RNA detection sensitivity; and reducing amplification bias. The present invention provides a method for nucleic acid amplification comprising a step for incubating a mixture including template RNA, primer, DNA strand-specific RNA:DNA hybrid strand decomposition enzyme, RNase H- reverse transcription enzyme, and substrate.

JP2017112958	NOVEL ALDEHYDE OXIDASE
WO/2010/013442	LIGHT-RESPONSIVE NUCLEOTIDE MOLECULE, AND METHOD FOR PRODUCTION OF LIGHT-RESPONSIVE MODIFIED NUCLEIC ACID BY USING THE LIGHT-RESPONSIVE NUCLEOTIDE MOLECULE
JPH04502705	[Title of the Invention] Attenuated virus

HAYASHI TETSUTARO (JP)
SASAGAWA YOHEI (JP)
NIKAIDO ITOSHI (JP)

PCT/JP2015/077745

April 07, 2016

September 30, 2015

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RIKEN (JP)

C12N15/09; C12N9/16; C40B40/06

JP2014103867A

2014-06-09

YOHEI SASAKAWA ET AL.: "Jisedai Genome Technology no Tojo to sono Mirai 6.1 Saibo RNA- Seq-ho no Saizensen to Kongo no Tenkai", EXPERIMENTAL MEDICINE, vol. 31, no. 15, 10 September 2013 (2013-09-10), pages 2358 - 2364, XP009501514
NILSEN, INGE W. ET AL.: "The Enzyme and the cDNA Sequence of a Thermolabile and Double-Strand Specific DNase from Northern Shrimps (Pandalus borealis", PLOS ONE, vol. 5, no. 4, April 2010 (2010-04-01), pages e10295, XP055424068
See also references of EP 3202901A4

SIKs & Co. (JP)
Patent business corporation patent firm Sykes (JP)

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