Title:
METHOD FOR PRODUCING CARRIER FOR CHROMATOGRAPHY, AND CARRIER FOR CHROMATOGRAPHY
Document Type and Number:
WIPO Patent Application WO/2024/029394
Kind Code:
A1
Abstract:
The present invention provides a carrier which is for chromatography, which has a large dynamic binding capacity with respect to an antibody or a fragment thereof, and through which a protein ligand is unlikely to leak even when the carrier is repeatedly used for isolation of an antibody. Provided is a method for producing a carrier for chromatography, said method comprising the following steps A-1 and B. (Step A-1) A step for immobilizing, to porous particles, one or more ligands selected from protein A, protein G, protein L, and analogues thereof. (Step B) A step for reacting the porous particles to which the one or more ligands have been immobilized in the step A-1 and a compound which has at least one ligand reactive group selected from groups represented by -C(=O)-O-C(=O)-, carbodiimide groups, and cyclic ether groups.
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Inventors:
INOUE YUKIYA (JP)
MIYAJIMA KEN (JP)
KAMIDE TOMOYUKI (JP)
NOGUCHI HIROSHI (JP)
NAKAMURA SATOSHI (JP)
MIYAJIMA KEN (JP)
KAMIDE TOMOYUKI (JP)
NOGUCHI HIROSHI (JP)
NAKAMURA SATOSHI (JP)
Application Number:
PCT/JP2023/027099
Publication Date:
February 08, 2024
Filing Date:
July 25, 2023
Export Citation:
Assignee:
JSR CORP (JP)
International Classes:
C07K1/22; B01D15/38; B01J20/24; B01J20/281; B01J20/30; C07K14/31; C07K16/00
Domestic Patent References:
| WO2020040307A1 | 2020-02-27 |
Foreign References:
| JP2018109643A | 2018-07-12 |
Other References:
PHILLIPS, T.M. ; QUEEN, W.D. ; MORE, N.S. ; THOMPSON, A.M.: "Protein A-coated glass beads", JOURNAL OF CHROMATOGRAPHY A, ELSEVIER, AMSTERDAM, NL, vol. 327, 26 June 1985 (1985-06-26), AMSTERDAM, NL, pages 213 - 219, XP026479835, ISSN: 0021-9673, DOI: 10.1016/S0021-9673(01)81651-4
ZHANG KUO; WANG LUNAN; LIU MIN; ZHANG RUI; LI JINMING: "Synthetic rabbit-human antibody conjugate as a control in immunoassays for immunoglobulin M specific to hepatitis E virus", VIROLOGY JOURNAL, BIOMED CENTRAL, LONDON, GB, vol. 7, no. 1, 20 May 2010 (2010-05-20), GB , pages 101, XP021080042, ISSN: 1743-422X, DOI: 10.1186/1743-422X-7-101
GRABAREK, Z. ; GERGELY, J.: "Zero-length crosslinking procedure with the use of active esters", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 185, no. 1, 15 February 1990 (1990-02-15), Amsterdam, NL , pages 131 - 135, XP024818117, ISSN: 0003-2697, DOI: 10.1016/0003-2697(90)90267-D
ZHANG KUO; WANG LUNAN; LIU MIN; ZHANG RUI; LI JINMING: "Synthetic rabbit-human antibody conjugate as a control in immunoassays for immunoglobulin M specific to hepatitis E virus", VIROLOGY JOURNAL, BIOMED CENTRAL, LONDON, GB, vol. 7, no. 1, 20 May 2010 (2010-05-20), GB , pages 101, XP021080042, ISSN: 1743-422X, DOI: 10.1186/1743-422X-7-101
GRABAREK, Z. ; GERGELY, J.: "Zero-length crosslinking procedure with the use of active esters", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, AMSTERDAM, NL, vol. 185, no. 1, 15 February 1990 (1990-02-15), Amsterdam, NL , pages 131 - 135, XP024818117, ISSN: 0003-2697, DOI: 10.1016/0003-2697(90)90267-D
Attorney, Agent or Firm:
ARUGA PATENT OFFICE (JP)
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