METHOD FOR PRODUCING CULTURE AND CELL COLLECTION METHOD

Title:

METHOD FOR PRODUCING CULTURE AND CELL COLLECTION METHOD

Document Type and Number:

WIPO Patent Application WO/2022/210659

Kind Code:

A1

Abstract:

A method for producing a culture, said method comprising: bringing adherent cells into contact with a soluble culture carrier that is larger in size than the adherent cells and thus placing the adherent cells on the surface of the soluble culture carrier; suspension-culturing in a medium the adherent cells that are placed on the surface of the soluble culture carrier; to release the adherent cells under the suspension culture from the surface of the soluble culture carrier, subjecting the soluble culture carrier to a denaturation treatment by which at least a part of the surface thereof is denatured; and, after the denaturation treatment, separating and collecting the adherent cells from the denatured soluble culture carrier that is larger in size than the adherent cells, depending on the size difference.

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Inventors:

SATO YUSHI (JP)
OKANOJO MASAHIRO (JP)
CHEN SHANGWU (JP)
TAKAHASHI RYOSUKE (JP)

Application Number:

PCT/JP2022/015315

Publication Date:

October 06, 2022

Filing Date:

March 29, 2022

Export Citation:

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Assignee:

SHOWA DENKO MATERIALS CO LTD (JP)

International Classes:

C12N5/02; C12N5/071; C12N5/0775

Domestic Patent References:

WO2014017513A1	2014-01-30
WO2015111686A1	2015-07-30

Foreign References:

JP2018068154A	2018-05-10
JPH02171183A	1990-07-02
JP2016523086A	2016-08-08
JP2021060494A	2021-04-15

Other References: