Title:
METHOD FOR PRODUCING INSULIN-PRODUCING CELLS
Document Type and Number:
WIPO Patent Application WO/2020/100789
Kind Code:
A1
Abstract:
The main problems addressed by this invention are the provision of: a method for producing insulin-producing cells from somatic cells without artificial gene transfer; insulin-producing cells obtained using said production method; and a composition containing a combination of chemical substances which can be used in said production method. This invention is, for example: a method for producing insulin-producing cells by direct inducing differentiation from somatic cells, characterized by including a step in which somatic cells are cultured in the presence of an RSK inhibitor; insulin-producing cells obtained by said production method; and a composition for producing insulin-producing cells from somatic cells by directly inducing differentiation, being a composition for producing insulin-producing cells by directly inducing differentiation from somatic cells, characterized by including an RSK inhibitor. The insulin-producing cells obtained by the present invention are useful in regenerative medicine and the like.
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Inventors:
DAI PING (JP)
TAKEDA YUKIMASA (JP)
HARADA YOSHINORI (JP)
MATSUMOTO JUNICHI (JP)
KUSAKA AYUMI (JP)
TAKEDA YUKIMASA (JP)
HARADA YOSHINORI (JP)
MATSUMOTO JUNICHI (JP)
KUSAKA AYUMI (JP)
Application Number:
PCT/JP2019/044058
Publication Date:
May 22, 2020
Filing Date:
November 11, 2019
Export Citation:
Assignee:
KATAOKA CORP (JP)
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
KYOTO PREFECTURAL PUBLIC UNIV CORP (JP)
International Classes:
C12N5/071; C12N5/077; C12N5/0775
Domestic Patent References:
| WO2018062269A1 | 2018-04-05 |
Foreign References:
| JP2002348243A | 2002-12-04 | |||
| CH3279951A |
Other References:
CHOUDHARY, AMIT ET AL.: "Quantitative-Proteomic Comparison of Alpha and Beta Cells to Uncover Novel Targets for Lineage Reprogramming", PLOS ONE, vol. 9, no. 4, 2014, pages 1 - 10, XP055707997, Retrieved from the Internet [retrieved on 20191125]
TANIOKA, TOSHIHIRO: "Molecular mechanisms of insulin resistance in beta cells", THE SHOWA UNIVERSITY JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 2, no. 2, 1 January 2011 (2011-01-01), JP, pages 127 - 138, XP009528083, ISSN: 1884-7854
HORI, YUICHI ET AL.: "Growth inhibitors promote differentiation of insulin-producing tissue from embryonic stem cells", PNAS, vol. 99, no. 25, 2002, pages 16105 - 16110, XP002392968, DOI: 10.1073/pnas.252618999
MA, XIAOJIE ET AL.: "Chemical strategies for pancreatic beta cell differentiation, reprogramming, and regeneration", ACTA BIOCHIM BIOPHYS SIN, vol. 49, no. 4, 2017, pages 289 - 301, XP055647725, DOI: 10.1093/abbs/gmx008
STEM CELLS INTERNATIONAL, vol. 2016
BIOMED RESEARCH INTERNATIONAL, vol. 2015
CURRENT PATHOBIOLOGY REPORTS, no. 3, 2015, pages 57 - 65
CELL STEM CELL, vol. 14, 2014, pages 228 - 236
NATURE COMMUNICATIONS, vol. 7, 2016, pages 10080
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 292, 2017, pages 19122 - 19132
PNAS, vol. 107, no. 34, 24 August 2010 (2010-08-24), pages 15099 - 15104
CAS, no. 1311367-27-7
CAS , no. 1620576-64-8
TANIOKA, TOSHIHIRO: "Molecular mechanisms of insulin resistance in beta cells", THE SHOWA UNIVERSITY JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 2, no. 2, 1 January 2011 (2011-01-01), JP, pages 127 - 138, XP009528083, ISSN: 1884-7854
HORI, YUICHI ET AL.: "Growth inhibitors promote differentiation of insulin-producing tissue from embryonic stem cells", PNAS, vol. 99, no. 25, 2002, pages 16105 - 16110, XP002392968, DOI: 10.1073/pnas.252618999
MA, XIAOJIE ET AL.: "Chemical strategies for pancreatic beta cell differentiation, reprogramming, and regeneration", ACTA BIOCHIM BIOPHYS SIN, vol. 49, no. 4, 2017, pages 289 - 301, XP055647725, DOI: 10.1093/abbs/gmx008
STEM CELLS INTERNATIONAL, vol. 2016
BIOMED RESEARCH INTERNATIONAL, vol. 2015
CURRENT PATHOBIOLOGY REPORTS, no. 3, 2015, pages 57 - 65
CELL STEM CELL, vol. 14, 2014, pages 228 - 236
NATURE COMMUNICATIONS, vol. 7, 2016, pages 10080
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 292, 2017, pages 19122 - 19132
PNAS, vol. 107, no. 34, 24 August 2010 (2010-08-24), pages 15099 - 15104
CAS, no. 1311367-27-7
CAS , no. 1620576-64-8
Attorney, Agent or Firm:
SHIMIZU Naoto (JP)
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