Title:
METHOD FOR PRODUCING STEM CELL-DERIVED LACRIMAL GLAND TISSUE
Document Type and Number:
WIPO Patent Application WO/2019/189640
Kind Code:
A1
Abstract:
This method is for producing stem cell-derived lacrimal gland tissue and is characterized by isolating cells that are positive to both SSEA4 and CD104 from a self-formed ectodermal autonomous multi-zone (SEAM) cell population derived from pluripotent stem cells, and three-dimensionally culturing the obtained cells in a culture medium containing epidermal growth factor (EGF) and a ROCK inhibitor, to obtain a cell population that expresses lacrimal gland-associated proteins. According to the present invention, a lacrimal gland organoid can be produced from pluripotent stem cells including iPS cells. Therefore, the present invention is very useful for regenerative therapies for lacrimal gland-related diseases using cellular medicine and studies related to the diseases.
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Inventors:
NISHIDA KOHJI (JP)
HAYASHI RYUHEI (JP)
OKUBO TORU (JP)
HONMA YOICHI (JP)
HAYASHI RYUHEI (JP)
OKUBO TORU (JP)
HONMA YOICHI (JP)
Application Number:
PCT/JP2019/013759
Publication Date:
October 03, 2019
Filing Date:
March 28, 2019
Export Citation:
Assignee:
UNIV OSAKA (JP)
International Classes:
C12N5/071; A61K35/30; A61K35/545; A61P27/02; A61P27/04; C12N5/10; C12Q1/02
Domestic Patent References:
| WO2016114285A1 | 2016-07-21 | |||
| WO2016153027A1 | 2016-09-29 | |||
| WO2016114285A1 | 2016-07-21 |
Other References:
LU Q. ET AL.: "An In Vitro Model for Ocular Surface and Tear Film System", SCIENTIFIC REPORTS, vol. 7, no. 6163, 21 July 2017 (2017-07-21), pages 1 - 11, XP055639215
KINOSHITA, S. ET AL.: "Characteristics of the Human Ocular Surface Epithelium", PROGRESS IN RETINAL AND EYE RESEARCH, vol. 20, no. 5, 2001, pages 639 - 673, XP002463578, DOI: 10.1016/S1350-9462(01)00007-6
NAGATO, SHOGO ET AL.: "Derivation of neuroepithelial cells from human iPS cells under three-dimensional culture using a microfluidic device", LECTURE ABSTRACTS OF THE 2016 42ND MEETING OF JAPANESE SOCIETY OF VETERINARY NEUROLOGY; JULY 16TH - 17TH, 2016, vol. 2016, 2016, pages 27 - 28, XP009523840
HIRAYAMA ET AL., NPJ AGING AND MECHANISMS OF DISEASE, vol. 3, no. 1, 2017
HAYASHI ET AL., NATURE PROTOCOLS, vol. 12, no. 4, 2017, pages 683 - 696
HAYASHI ET AL., NATURE, vol. 531, no. 7594, 17 March 2016 (2016-03-17), pages 376 - 80
See also references of EP 3778871A4
KINOSHITA, S. ET AL.: "Characteristics of the Human Ocular Surface Epithelium", PROGRESS IN RETINAL AND EYE RESEARCH, vol. 20, no. 5, 2001, pages 639 - 673, XP002463578, DOI: 10.1016/S1350-9462(01)00007-6
NAGATO, SHOGO ET AL.: "Derivation of neuroepithelial cells from human iPS cells under three-dimensional culture using a microfluidic device", LECTURE ABSTRACTS OF THE 2016 42ND MEETING OF JAPANESE SOCIETY OF VETERINARY NEUROLOGY; JULY 16TH - 17TH, 2016, vol. 2016, 2016, pages 27 - 28, XP009523840
HIRAYAMA ET AL., NPJ AGING AND MECHANISMS OF DISEASE, vol. 3, no. 1, 2017
HAYASHI ET AL., NATURE PROTOCOLS, vol. 12, no. 4, 2017, pages 683 - 696
HAYASHI ET AL., NATURE, vol. 531, no. 7594, 17 March 2016 (2016-03-17), pages 376 - 80
See also references of EP 3778871A4
Attorney, Agent or Firm:
IWATANI, Ryo (JP)
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