Title:
METHOD FOR PRODUCING SUGAR
Document Type and Number:
WIPO Patent Application WO/2012/077684
Kind Code:
A1
Abstract:
The present invention relates to a method for producing a sugar by saccharifying a cellulose having a cellulose I type crystallinity, as represented by equation (1), of 50% or lower and/or a specific surface area of 180 m2/g or higher, by means of an enzyme formulation, and relates to a method for increasing the quantity of glucose produced, wherein the enzyme formulation contains 20% or more of endoglucanase relative to the total quantity of proteins in the enzyme component, and the cellulose is obtained by pulverizing a cellulose-containing raw material in a pulverizer. Cellulose I type crystallinity (%) = [(I22.6 - I18.5) / I22.6] × 100 (1) (I22.6 denotes the diffraction intensity of the lattice plane (002 plane) (diffraction angle (2θ) = 22.6°) and I18.5 denotes the diffraction intensity of the amorphous portion (diffraction angle (2θ) = 18.5°) in X-ray diffraction)
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Inventors:
KAWANO TAKAKO (JP)
OGAWA AKINORI (JP)
SAITO KAZUHIRO (JP)
OGAWA AKINORI (JP)
SAITO KAZUHIRO (JP)
Application Number:
PCT/JP2011/078204
Publication Date:
June 14, 2012
Filing Date:
December 06, 2011
Export Citation:
Assignee:
KAO CORP (JP)
KAWANO TAKAKO (JP)
OGAWA AKINORI (JP)
SAITO KAZUHIRO (JP)
KAWANO TAKAKO (JP)
OGAWA AKINORI (JP)
SAITO KAZUHIRO (JP)
International Classes:
C12P19/14; C12N9/42; C12N15/09
Domestic Patent References:
WO2010134560A1 | 2010-11-25 |
Foreign References:
JP2009171952A | 2009-08-06 | |||
JP2009171951A | 2009-08-06 |
Other References:
GAO, D. ET AL.: "Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover", BIORESOUR. TECHNOL., vol. 101, no. 8, April 2010 (2010-04-01), pages 2770 - 2781, XP002591486, DOI: doi:10.1016/j.biortech.2009.10.056
ZHOU, J. ET AL.: "Optimization of cellulase mixture for efficient hydrolysis of steam- exploded corn stover by statistically designed experiments", BIORESOUR.TECHNOL., vol. 100, no. 2, 2009, pages 819 - 825
RAMAKRISHNAN, S. ET AL.: "Enzymatic hydrolysis of cellulose dissolved in N-methyl morpholine oxide/water solutions", BIORESOUR.TECHNOL., vol. 101, no. 13, July 2010 (2010-07-01), pages 4965 - 4970, XP026986236
ZHOU, J. ET AL.: "Optimization of cellulase mixture for efficient hydrolysis of steam- exploded corn stover by statistically designed experiments", BIORESOUR.TECHNOL., vol. 100, no. 2, 2009, pages 819 - 825
RAMAKRISHNAN, S. ET AL.: "Enzymatic hydrolysis of cellulose dissolved in N-methyl morpholine oxide/water solutions", BIORESOUR.TECHNOL., vol. 101, no. 13, July 2010 (2010-07-01), pages 4965 - 4970, XP026986236
Attorney, Agent or Firm:
OHTANI, Tamotsu et al. (JP)
Tamotsu Otani (JP)
Tamotsu Otani (JP)
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