Title:
METHOD AND SYSTEM FOR DETECTING BACTERIA IN SURGICAL CLEANING SOLUTION
Document Type and Number:
WIPO Patent Application WO/2013/021894
Kind Code:
A1
Abstract:
Provided are a method and a system, each of which can detect the presence of bacteria in a surgical cleaning solution containing a biological sample in a simple manner. The present invention relates to a method for detecting bacteria in a surgical cleaning solution, comprising: (i) mixing the surgical cleaning solution with a platelet-activating substance to allow the surgical cleaning solution and the platelet-activating substance to contact with each other; (ii) removing the platelet-activating substance from the surgical cleaning solution to produce a sample of interest; (iii) reacting the sample of interest with a C factor-containing reagent that can be activated through the binding to an endotoxin contained in the sample of interest and a luminescent synthetic substrate to cause the release of the luminescent substrate from the luminescent synthetic substrate; (iv) allowing a luminescent enzyme to act on the luminescent substrate that has been released in the above-mentioned step (iii) and measuring the quantity of emitted light; and (v) comparing the quantity of emitted light obtained in the above-mentioned step (iv) with a reference value.
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Inventors:
KIMURA KYOUTA (JP)
KOYAMA MIYUKI (JP)
KOYAMA MIYUKI (JP)
Application Number:
PCT/JP2012/069584
Publication Date:
February 14, 2013
Filing Date:
August 01, 2012
Export Citation:
Assignee:
TERUMO CORP (JP)
KIMURA KYOUTA (JP)
KOYAMA MIYUKI (JP)
KIMURA KYOUTA (JP)
KOYAMA MIYUKI (JP)
International Classes:
G01N33/48; G01N21/76; G01N33/579
Domestic Patent References:
| WO2009063840A1 | 2009-05-22 |
Foreign References:
| JP2007064895A | 2007-03-15 | |||
| JP2006284606A | 2006-10-19 |
Other References:
MASAO TOMITA ET AL.: "Detection of bacteria in lavage fluid of operative field during surgery and their sensitivity to cephem drugs", PROG MED, vol. 5, no. 11, November 1985 (1985-11-01), pages 3071 - 3076
KEN'ICHI NODA ET AL.: "Endotoxin Assay by Bioluminescence Using Mutant Firefly Luciferase", JAPAN JOURNAL OF CRITICAL CARE FOR ENDOTOXEMIA, vol. 15, 31 October 2011 (2011-10-31), pages 44 - 50
KEN'ICHI NODA ET AL.: "Endotoxin Assay by Bioluminescence Using Mutant Firefly Luciferase", JAPAN JOURNAL OF CRITICAL CARE FOR ENDOTOXEMIA, vol. 15, 31 October 2011 (2011-10-31), pages 44 - 50
Attorney, Agent or Firm:
HATTA & ASSOCIATES (JP)
Hatta international patent business corporation (JP)
Hatta international patent business corporation (JP)
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