Title:
MODIFIED ΑLPHA-GLUCOSIDASE AND APPLICATIONS OF SAME
Document Type and Number:
WIPO Patent Application WO/2012/124520
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing an α-glucosidase with dominant transglycosylation activity, and applications of the α-glucosidase. This modified α-glucosidase comprises an amino acid sequence in which one or more of the amino acids selected from the following groups have been substituted for another amino acid in the amino acid sequence of an α-glucosidase: the amino acid corresponding to amino acid position 385, 491, 535, 450, 534, 537, 538, 554, 556, 630, 683 or 689 of the amino acid sequence represented by SEQ ID NO: 1, or the amino acid corresponding to amino acid position 579 or 585 of the amino acid sequence represented by SEQ ID NO: 2.
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Inventors:
ISHIHARA SATORU (JP)
Application Number:
PCT/JP2012/055558
Publication Date:
September 20, 2012
Filing Date:
March 05, 2012
Export Citation:
Assignee:
AMANO ENZYME INC (JP)
ISHIHARA SATORU (JP)
ISHIHARA SATORU (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/24; C12P19/16; C12P19/18
Domestic Patent References:
| WO2010010463A2 | 2010-01-28 |
Foreign References:
| JP2001302440A | 2001-10-31 | |||
| JP2009022267A | 2009-02-05 | |||
| JP2002531581A | 2002-09-24 | |||
| JP2003088365A | 2003-03-25 | |||
| JP2005253302A | 2005-09-22 | |||
| JP2009022204A | 2009-02-05 | |||
| JP2001046096A | 2001-02-20 |
Other References:
MARIKO NISHIMURA ET AL.: "Schwanniomyces occidentalis Yurai GH31 a-glucosidase eno Hen'i Donyu ni yoru Glucoside Ketsugo Sentakusei no Henkan", JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY 2008 NENDO (HEISEI 20 NENDO) TAIKAI KOEN YOSHISHU, vol. 2A09A13, 2008, pages 33, XP008167193
MASAYUKI OKUYAMA: "Glycosidase that lost hydrolytic activity catalyzes oligosaccharide synthesis. Efficient synthesis of oligosaccharides by glycosynthase", KAGAKU TO SEIBUTSU, vol. 41, no. 7, 2003, pages 422 - 425, XP008170938
HIRONORI HONDO ET AL.: "Dextran glucosidase no Kasui Bunkai Oyobi To Ten'i Hanno ni Okeru Kishitsu Tokuisei no Henkan", J.APPL.GLYCOSCI., vol. 55, 2008, pages 62, S-6, XP008170883
SATO F. ET AL.: "Glucoamylase originating from Schwanniomyces occidentalis is a typical alpha-glucosidase.", BIOSCI.BIOTECHNOL.BIOCHEM., vol. 69, no. 10, 6 October 2005 (2005-10-06), pages 1905 - 1913, XP055125361
NAKAI H. ET AL.: "Molecular analysis of alpha- glucosidase belonging to GH-family 31.", BIOLOGIA, BRATISLAVA, vol. 60, no. 16, 2005, pages 131 - 135, XP055125363
NICHOLS B.L. ET AL.: "Human small intestinal maltase-glucoamylase cDNA cloning. Homology to sucrase-isomaltase.", J.BIOL.CHEM., vol. 273, no. 5, 30 January 1998 (1998-01-30), pages 3076 - 81, XP002449287
"Molecular Cloning", COLD SPRING HARBOR LABORATORY PRESS
FREDERICK M. AUSUBEL ET AL.,: "Current protocols in molecular biology", 1987
J. MOL. BIOL., vol. 53, 1970, pages 159
J. MOL. BIOL., vol. 166, 1983, pages 557
GENE, vol. 96, 1990, pages 23
CHUNG ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 86, 1989, pages 2172
POTTER, H. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 7161 - 7165
FELGNER, P.L. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1984, pages 7413 - 7417
J. MOL. BIOL., vol. 331, 2003, pages 585 - 592
NUCLEIC ACID RES., vol. 16, 1988, pages 7351 - 7367
SHIMIZU, Y. ET AL., NATURE BIOTECH., vol. 19, 2001, pages 751 - 755
PAVELKA ET AL., NUCLEIC ACIDS RES., vol. 37, 2009, pages W376 - 83
MASAYUKI OKUYAMA: "Glycosidase that lost hydrolytic activity catalyzes oligosaccharide synthesis. Efficient synthesis of oligosaccharides by glycosynthase", KAGAKU TO SEIBUTSU, vol. 41, no. 7, 2003, pages 422 - 425, XP008170938
HIRONORI HONDO ET AL.: "Dextran glucosidase no Kasui Bunkai Oyobi To Ten'i Hanno ni Okeru Kishitsu Tokuisei no Henkan", J.APPL.GLYCOSCI., vol. 55, 2008, pages 62, S-6, XP008170883
SATO F. ET AL.: "Glucoamylase originating from Schwanniomyces occidentalis is a typical alpha-glucosidase.", BIOSCI.BIOTECHNOL.BIOCHEM., vol. 69, no. 10, 6 October 2005 (2005-10-06), pages 1905 - 1913, XP055125361
NAKAI H. ET AL.: "Molecular analysis of alpha- glucosidase belonging to GH-family 31.", BIOLOGIA, BRATISLAVA, vol. 60, no. 16, 2005, pages 131 - 135, XP055125363
NICHOLS B.L. ET AL.: "Human small intestinal maltase-glucoamylase cDNA cloning. Homology to sucrase-isomaltase.", J.BIOL.CHEM., vol. 273, no. 5, 30 January 1998 (1998-01-30), pages 3076 - 81, XP002449287
"Molecular Cloning", COLD SPRING HARBOR LABORATORY PRESS
FREDERICK M. AUSUBEL ET AL.,: "Current protocols in molecular biology", 1987
J. MOL. BIOL., vol. 53, 1970, pages 159
J. MOL. BIOL., vol. 166, 1983, pages 557
GENE, vol. 96, 1990, pages 23
CHUNG ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 86, 1989, pages 2172
POTTER, H. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 81, 1984, pages 7161 - 7165
FELGNER, P.L. ET AL., PROC. NATL. ACAD. SCI. U.S.A., vol. 84, 1984, pages 7413 - 7417
J. MOL. BIOL., vol. 331, 2003, pages 585 - 592
NUCLEIC ACID RES., vol. 16, 1988, pages 7351 - 7367
SHIMIZU, Y. ET AL., NATURE BIOTECH., vol. 19, 2001, pages 751 - 755
PAVELKA ET AL., NUCLEIC ACIDS RES., vol. 37, 2009, pages W376 - 83
Attorney, Agent or Firm:
HAGINO Mikiharu (JP)
Kanji Hagino (JP)
Kanji Hagino (JP)
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