NOVEL PROMOTER HASP1 OF PHAEODACTYLUM TRICORNUTUM AND SIGNAL PEPTIDE THEREOF, AND USE THEREOF

WIPO Patent Application WO/2021/002630

A1

The present invention relates to a novel promoter HASP1 for inducing overexpression of a protein in Phaeodactylum tricornutum; a signal peptide HASP1-SP, which is a novel protein secretion sequence for inducing the secretion of the expressed protein; an expression vector comprising the HASP1 and the HASP1-SP; a transformant in which the expression vector has been introduced into a host cell; a method for producing a protein of interest comprising a step of culturing the transformant to obtain a culture; a method for expressing a protein of interest comprising a step of transforming the expression vector into a host cell; and a method for increasing the expression level of a protein of interest in a host cell comprising the step of transforming the expression vector into the host cell. The present invention is expected to be used as a powerful genetic tool capable of significantly increasing the productivity of a recombinant protein by using a novel promoter HASP1 for inducing overexpression of a protein in Phaeodactylum tricornutum , a signal peptide HASP1-SP which is a novel protein secretion sequence for inducing the secretion of the expressed protein, and an expression vector comprising the HASP1 and the HASP1-SP according to the present invention.

WO/1996/040713	CELLULAR APOPTOSIS SUSCEPTIBILITY PROTEIN (CSP) AND ANTISENSE CSP
WO/2001/092530	NUCLEIC ACID MOLECULE COMPRISING A NUCLEIC ACID SEQUENCE CODING FOR AN SDF-1 GAMMA CHEMOKINE, A NEUROPEPTIDE PRECURSOR OR AT LEAST ONE NEUROPEPTIDE
WO/1995/018854	RIBOZYMES TARGETING THE RETROVIRAL PACKAGING SEQUENCE EXPRESSION CONSTRUCTS AND RECOMBINANT RETROVIRUSES CONTAINING SUCH CONSTRUCTS

PAN CHEOL-HO (KR)
ERDENEDOLGOR ERDENE-OCHIR (KR)
SONG DAE-GEUN (KR)
SHIN BOK-KYU (KR)
KWON BYEORI (KR)

PCT/KR2020/008251

January 07, 2021

June 25, 2020

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KOREA INST SCI & TECH (KR)
ALGAEPRONA INC (KR)

C12N15/79; C07K7/08; C12N15/67; C12P21/00

KR20180050237A

2018-05-14

DATABASE Nucleotide ANONYMOUS: "Phaeodactylum tricornutum CCAP 1055/1 predicted protein mRNA", XP055771185, retrieved from NCBI Database accession no. XM_002181840.1
MICHAEL ADAN CABALLERO: "Investigating the biochemistry and genetics of chrysolaminarin metabolism in a model marine diatom", THESES AND DISSERTATIONS, 1 January 2007 (2007-01-01), pages 1 - 178, XP055771175
LIOUDMILA A ZASLAVSKAIA , J CASEY LIPPMEIER , PETER G KROTH , ARTHUR R GROSSMAN , KIRK E APT: "Transformation of the diatom Phaeodactylum tricornutum (Bacillariophyceae) with a variety of selectable marker and reporter genes", JOURNAL OF PHYCOLOGY, vol. 36, no. 2, 1 April 2000 (2000-04-01), pages 379 - 386, XP002628990, ISSN: 0022-3646, DOI: 10.1046/j.1529-8817.2000.99164.x
SEUNGBEOM SEO, JEON HANCHEOL, HWANG SEONGBIN, JIN EONSEON, CHANG KWANG SUK: "Development of a new constitutive expression system for the transformation of the diatom Phaeodactylum tricornutum", ALGAL RESEARCH, vol. 11, 1 September 2015 (2015-09-01), pages 50 - 54, XP055771161, ISSN: 2211-9264, DOI: 10.1016/j.algal.2015.05.012
ERDENEDOLGOR ERDENE-OCHIR, SHIN BOK-KYU, KWON BYEORI, JUNG CHOONKYUN, PAN CHEOL-HO: "Identification and characterisation of the novel endogenous promoter HASP1 and its signal peptide from Phaeodactylum tricornutum", SCIENTIFIC REPORTS, vol. 9, no. 1, 9941, 9 July 2019 (2019-07-09), pages 1 - 10, XP055771154, ISSN: 2045-2322, DOI: 10.1038/s41598-019-45786-9
"NCBI", Database accession no. XM_002181840.1

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