POLYPEPTIDE, FUSED POLYMER PROTEIN AND USE THEREOF

WIPO Patent Application WO/2024/045430

A1

The present invention relates to the technical field of biological functions of proteins, and specifically relates to a polypeptide, a fused polymer protein and the use thereof. The polypeptide is selected from: (1) a polypeptide that has a substitution mutation in at least one position selected from positions 16, 25, 29, 49 and 58 compared to the native C domain of protein A as shown in SEQ ID NO. 1, wherein the amino acid at position 16 is subjected to substitution mutation to leucine or valine, the amino acid at position 25 is subjected to substitution mutation to lysine, arginine, histidine or tryptophan, the amino acid at position 29 is subjected to substitution mutation to alanine, leucine or threonine, the amino acid at position 49 is subjected to substitution mutation to arginine or histidine, and the amino acid at position 58 is subjected to substitution mutation to glycine, isoleucine or alanine; and (2) a polypeptide that has at least 80% or more homology to the polypeptide in (1) and retains a substitution mutation in at least one of positions 16, 25, 29, 49 and 58. The polypeptide has high alkali tolerance and high loading capacity.

WO/2005/108579	POLYNUCLEOTIDES FOR THE DETECTION OF STAPHYLOCOCCUS AUREUS
JP2000316574	DETECTION OF TOXOPROTEIN PRODUCED BY MRSA, MONOCLONAL ANTIBODY AND ITS FRAGMENT, KIT FOR DETECTING TOXOPROTEIN PRODUCED BY MRSA AND HYBRIDOMA CAPABLE OF PRODUCING MONOCLONAL ANTIBODY
WO/1999/032133	METHODS AND COMPOSITIONS FOR THE TREATMENT AND PREVENTION OF $i(STAPHYLOCOCCUS AUREUS) INFECTIONS

LI YANJUN (CN)
LI GANG (CN)
GU TONGNIAN (CN)
ZHANG JIANTAO (CN)
LIU LONG (CN)
LEI XIAOJU (CN)
LIU QIONG (CN)

PCT/CN2022/140536

March 07, 2024

December 21, 2022

Click for automatic bibliography generation  

SUNRESIN NEW MAT CO LTD (CN)

C07K14/31; B01D15/38; C07K1/22; C07K16/00; C07K19/00; C12N1/21; C12N15/31; C12N15/62; C12N15/70; G01N33/68; C12R1/19

CN114149495A	2022-03-08
CN112639099A	2021-04-09
CN105377880A	2016-03-02
CN102895960A	2013-01-30
CN102365361A	2012-02-29
US20170327534A1	2017-11-16
CN103443120A	2013-12-11
CN114280208A	2022-04-05

VINOD AMRITKAR ET AL.: "Engineering Staphylococcal Protein A for High-throughput Affinity Purification of Monoclonal Antibodies", BIOTECHNOLOGY ADVANCES, vol. 44, 22 September 2020 (2020-09-22), XP086282857, DOI: 10.1016/j.biotechadv.2020.107632
DUGGLEBY, C.JJONES, SA: "cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli", NUCL ACIDS RES, vol. 11, 1983, pages 3065 - 3076, XP009189641
LOFDAHL, S.GUSS, B. ET AL.: "Gene for Staphylococcal protein A", PROC. NATL. ACID. SCI. USA, vol. 80, no. 1983, pages 697 - 701, XP008095034, DOI: 10.1073/pnas.80.3.697
JANSSON BUHLEN MNYGREN PA: "All individual domains of staphylococcal protein A show Fab binding", FEMS IMMUNOL MED MICROBIOL, vol. 20, no. 1, January 1998 (1998-01-01), pages 69 - 78, XP002284946, DOI: 10.1016/S0928-8244(97)00108-9
GARSHASBRIGISAMIRA ET AL.: "A comprehensive review on staphylococcal protein A (SpA): Its production and applications", BIOTECHNOLOGY & APPLIED BIOCHEMISTRY, 2019
AMRITKAR VADAT STEJWANI V ET AL.: "Engineering Staphylococcal Protein A for high-throughput affinity purification of monoclonal antibodies", BIOTECHNOLOGY ADVANCES, 2020, pages 107632

SUNSHINE INTELLECTUAL PROPERTY INTERNATIONAL CO., LTD. (CN)

View/Download PDF      PDF Help

Previous Patent: PERFORMANCE TEST METHOD FOR FULL-AUTOMATIC SAMPLE PREPARATION SYSTEM

Next Patent: ZEIN FIBRIL POWDER, ZEIN NEW GEL, AND PREPARATION METHODS THEREFOR AND USES THEREOF