Title:
SIGNAL COUNTING FOR IN SITU HYBRIDIZATION
Document Type and Number:
WIPO Patent Application WO2001020044
Kind Code:
A3
Abstract:
Fluorescently tagged nucleic acid probe signals are counted in biological specimens by determining a ratio of signals from a test probe to signals of a reference probe. Probe signals need not be counted with reference to cells, nuclei, or nuclear contours. Gene amplification or deletion can thus be detected by analyzing the ratio. Successive image slices are obtained by confocal microscopy, and the images are digitized. The digital images are transformed and analyzed to combine contiguous fluorescent signal segments in successive optical sections to identify discrete probe signals, or spots. Spots overlapping in the axial and transverse dimensions of a three-dimensional representation of the biological specimens can be distinguisghed. A graphical user interface presents various features for consideration by a user, who can provide guidance to a computer system counting the spots. Various features directed to identifying spot clusters and autofluorescent material can increase accuracy of spot counting.
More Like This:
Inventors:
KALLIONIEMI OLLI P (US)
KONONEN JUHA (US)
BUDENDORF LUKAS (US)
DOUGHERTY EDWARD R (US)
GRIGORYAN ARTYOM M (US)
KONONEN JUHA (US)
BUDENDORF LUKAS (US)
DOUGHERTY EDWARD R (US)
GRIGORYAN ARTYOM M (US)
Application Number:
PCT/US2000/025465
Publication Date:
May 10, 2002
Filing Date:
September 15, 2000
Export Citation:
Assignee:
US HEALTH (US)
KALLIONIEMI OLLI P (US)
KONONEN JUHA (US)
BUDENDORF LUKAS (US)
DOUGHERTY EDWARD R (US)
GRIGORYAN ARTYOM M (US)
KALLIONIEMI OLLI P (US)
KONONEN JUHA (US)
BUDENDORF LUKAS (US)
DOUGHERTY EDWARD R (US)
GRIGORYAN ARTYOM M (US)
International Classes:
C12Q1/68; C12Q1/6809; C12Q1/6816; C12Q1/6841; G01N15/14; (IPC1-7): C12Q1/68; G01N15/14
Domestic Patent References:
| WO1999044063A2 | 1999-09-02 |
Foreign References:
| US5817462A | 1998-10-06 |
Other References:
RODENACKER ET AL.: "Groping for quantitative digital 3-D image analysis: an approach to quantitative fluorescence in situ hybridization in thick tissue sections of prostate carcinoma", ANALYTICAL CELLULAR PATHOLOGY, vol. 15, no. 1, 1997, pages 19 - 29, XP001024113
AUBELE ET AL.: "Comparative FISH analysis of numerical chromosome 7 abnormalities in 5-um and 15-um paraffin-embedded tissue sections from prostatic carcinoma", HISTOCHEMISTRY AND CELL BIOLOGY, vol. 107, no. 2, 1997, pages 121 - 126, XP001024090
AUBELE ET AL.: "Comparative FISH analysis of numerical chromosome 7 abnormalities in 5-um and 15-um paraffin-embedded tissue sections from prostatic carcinoma", HISTOCHEMISTRY AND CELL BIOLOGY, vol. 107, no. 2, 1997, pages 121 - 126, XP001024090
Download PDF:
Previous Patent: METHOD OF CLUSTER ANALYSIS OF GENE EXPRESSION PROFILES
Next Patent: COPPER COOLING PLATE FOR METALLURGICAL FURNACES
Next Patent: COPPER COOLING PLATE FOR METALLURGICAL FURNACES