SPECIFIC AND SENSITIVE METHOD FOR AMPLIFYING TARGET SEQUENCE

WIPO Patent Application WO/2007/074894

A1

It is intended to develop a method for reducing a noise from a non-target gene having a similar sequence and amplifying only a target gene and a novel quantitative method by obtaining a signal from the amplified gene with high accuracy (high sensitivity and high specificity). As a result of intensive studies made by the inventors of the invention, it was found that the object can be achieved by incorporating a third primer in a PCR reaction mixture, thus the invention has been completed.

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MURAYAMA OHOSHI (JP)
UENO HITOMI (JP)

PCT/JP2006/326200

July 05, 2007

December 28, 2006

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SCHOOL CORP AZABU VETERINARY M (JP)
MURAYAMA OHOSHI (JP)
UENO HITOMI (JP)

C12Q1/68; C12N15/09

WO1998049351A1

1998-11-05

JP2003159100A	2003-06-03
JP2002503089A	2002-01-29
JPH05292968A	1993-11-09

MURAYAMA Y.: "Bishokan Ketsugo Protein Tau no Shinkei Saiboshi ni Okeru Yakuwari ni Kansuru Kenkyu", JOURNAL OF AZABU UNIVERSITY, vol. 9-10, 31 March 2005 (2005-03-31), pages 146 - 147, XP003015045
THOMSON K.G. ET AL.: "Identification of Zucchini yellow mosaic potyvirus by RT-PCR and analysis of sequence variability", J. VIROL. METHODS, vol. 55, no. 1, September 1995 (1995-09-01), pages 83 - 96, XP003015046

SHAMOTO, Ichio et al. (Section 206 New Ohtemachi Bldg., 2-1, Ohtemachi 2-chom, Chiyoda-ku Tokyo 04, JP)

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