Title:
TRANSGENIC SILKWORM CAPABLE OF PRODUCING FUNCTIONAL ANTIBODY MOLECULE
Document Type and Number:
WIPO Patent Application WO/2008/032748
Kind Code:
A1
Abstract:
Disclosed is a novel means for producing a recombinant antibody at low cost and
in a large quantity. Specifically disclosed is a transgenic silkworm in which
a structural gene for each of an H-chain and an L-chain of an antibody is integrated
in the chromosome. In the transgenic silkworm, two H-chain molecules and two
L-chain molecules are linked via a disulfide bond. The transgenic silkworm can
secrete a functional antibody molecule having an activity of binding to an antigen
into a cocoon. Also disclosed is a method for producing an antibody, which comprises
collecting the functional antibody molecule from a cocoon of the silkworm.
Inventors:
OGAWA SHINGO (JP)
TOMITA MASAHIRO (JP)
SHIMIZU KATSUHIKO (JP)
YOSHIZATO KATSUTOSHI (JP)
TOMITA MASAHIRO (JP)
SHIMIZU KATSUHIKO (JP)
YOSHIZATO KATSUTOSHI (JP)
Application Number:
PCT/JP2007/067758
Publication Date:
March 20, 2008
Filing Date:
September 12, 2007
Export Citation:
Assignee:
HIROSHIMA IND PROMOTION ORG (JP)
NEOSILK CO LTD (JP)
UNIV HIROSHIMA (JP)
OGAWA SHINGO (JP)
TOMITA MASAHIRO (JP)
SHIMIZU KATSUHIKO (JP)
YOSHIZATO KATSUTOSHI (JP)
NEOSILK CO LTD (JP)
UNIV HIROSHIMA (JP)
OGAWA SHINGO (JP)
TOMITA MASAHIRO (JP)
SHIMIZU KATSUHIKO (JP)
YOSHIZATO KATSUTOSHI (JP)
International Classes:
C12N15/00; A01K67/033; C07K16/00; C12N15/09
Foreign References:
| JP2006109772A | 2006-04-27 | |||
| JP2004016144A | 2004-01-22 | |||
| JP2004135528A | 2004-05-13 | |||
| JP2004344123A | 2004-12-09 | |||
| JP2005095063A | 2005-04-14 | |||
| JP2006016323A | 2006-01-19 |
Other References:
TOMITA M. ET AL.: "Transgenic silkworms produce recombinant human type III procollagen in cocoons", NATURE BIOTECH., vol. 21, no. 1, January 2003 (2003-01-01), pages 52 - 56, XP002975379
GUTTIERI M.C. ET AL.: "Cassete Vectors for conversion of Fab fragments into full-length human IgG1 monoclonal antibodies by expression in stably transformed insect cells", HYBRIDOMA HYBRIDOMMICS, vol. 22, no. 3, June 2003 (2003-06-01), pages 135 - 145, XP009083949
GUTTIERI M.C. ET AL.: "Cassete Vectors for conversion of Fab fragments into full-length human IgG1 monoclonal antibodies by expression in stably transformed insect cells", HYBRIDOMA HYBRIDOMMICS, vol. 22, no. 3, June 2003 (2003-06-01), pages 135 - 145, XP009083949
Attorney, Agent or Firm:
NISHIZAWA, Toshio (3-14 Kudan-kita 4-chom, Chiyoda-ku Tokyo 73, JP)
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