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JP2010131005A |
To provide a repeatedly available immobilized biocatalyst which can perform efficient separating and collection with simple operation from a reaction solution containing products without collecting a residual material such as a water-ins...
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JP4467793B2 |
A technique for controlling membrane denaturation reactions other than physical shear force was developed. For example, the present invention provides, a method for causing membrane disruption at a specific site by reacting a stimulus su...
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JP4455801B2 |
Methods and devices for the regulation of type II collagen gene expression in cartilage cells via the application of specific and selective fields generated by specific and selective electric and electromagnetic signals in the treatment ...
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JP4451929B2 |
Methods and support systems are provided for modifying the regeneration, differentiation, or function of cells. In one embodiment, electrically conducting biocompatible polymers may be used alone or in combination with a polymeric suppor...
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JP2010063459A |
To provide a method and a support system for modifying regeneration, differentiation or function of a cell.There are provided an electrically conducting biocompatible polymer which may be used alone or in combination with a polymeric sup...
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JP4440881B2 |
Epidermal keratinocytes not incorporating magnetic particles therein and epidermal keratinocytes incorporating magnetic particles therein were seeded in a 24-well ultra-low-attachment plate, and cultured for one day after a neodymium mag...
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JP2010029193A |
To provide a method for removing existing tumor cells from a cell population.Provided is an in vitro method for removing tumor cells from a cell population containing non-tumor cells comprising the labeling of individual tumor cells in t...
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JP2010022285A |
To provide a method for effectively destructing biological cells by applying an alternate magnetic field from the outside.A cell-containing solution is injected with ultraparticulate ferromagnetic ferrite of high magnetic permeability fo...
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JP4380857B2 |
To provide a method for realizing gene expression in arbitrary local region. This method comprises irradiating infrared laser selectively to local region such as a single cell or an organ of bion increasing the temperature of the local r...
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JP2009538694A |
Methods and devices are described for the regulation of Transforming Growth actor (TGF)-beta1, beta2, and/or beta3 protein gene expression in bone cells and other tissues via the capacitive coupling or inductive coupling of specific and ...
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JP4353582B2 |
The opening is introduced into the cell (3) membrane within the area of the cell mounting (5) and spaced away from the support edge An Independent claim is included for suitable equipment which includes the cell mounting with e.g. approp...
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JP4344321B2 |
Methods and devices for the regulation of aggrecan gene expression in cartilage cells via the application of fields generated by specific and selective electric and electromagnetic signals in the treatment of diseased or injured articula...
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JP2009195110A |
To carry out a treatment of cells except specific cells without damaging the specific cells.An image-treatment device 12 acquires images of cell groups containing a plurality of the cells present in a culture container 38 at a prescribed...
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JP4326343B2 |
A molecule vibration apparatus comprises a voltage generation means, an electrode which generates instantaneous high electric fields on specified positions on tissues or biological organisms upon receiving the voltage, and an electric fi...
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JP4321962B2 |
The invention relates to a method for permeabilization of a cell structure consisting of a single cell, an intracellular structure or an organelle comprising the following steps: (a) microelectrodes, preferably two carbon fiber electrode...
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JP4307255B2 |
Methods are disclosed for sterilizing preparations of digestive enzymes to reduce the level of one or more active biological contaminants or pathogens therein, such as viruses, bacteria (including inter- and intracellular bacteria, such ...
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JP4282823B2 |
A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of th...
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JP4285933B2 |
Anaerobically decomposed organic materials such as landfill material or sewage sludge are treated by passage of an electric current therethrough in order to enhance production of hydrogen, suppress formation of methane and decrease the t...
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JP4264134B2 |
The method of processing biopolymers, microorganisms and substances by using two or more kinds of magnetic particles according to this invention can automatically and consistently perform a series of processes, such as extraction, isolat...
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JP2009082144A |
To provide rapid and efficient injection of various foreign molecules to a cell, having a high cell survival rate.An optoinjection method allows a target cell to be transiently permealized by (a) a process for irradiating a population of...
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JP2009065914A |
To provide a method for regulating gene expression associated with a hematopoietic stem cell, and to provide a method for differentiating-amplifying the hematopoietic stem cell.The method for regulating the gene expression includes carry...
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JP2009034114A |
To provide methods and support systems for modifying the regeneration, differentiation, or function of cells.In one embodiment, electrically conducting biocompatible polymers may be used alone or in combination with a polymeric support f...
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JP2009506756A |
The present invention relates to a method of treating cells with an electric field using lipophilic ions as a medium additive in order to change the membrane permeability of the cells. In particular, the present invention relates to an e...
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JP2009022295A |
To provide a method for rapidly and effectively identifying and targeting specific cells found in a compound specimen in a biological tissue.The invention provides a device for electromagnetically affecting a particle of interest in a sp...
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JP4217618B2 |
An object of the invention is to provide a method of treating biological cells prior to subjecting the biological cells to cell fusion pulses which includes the step of treating the biological cells with pre-fusion electric field wavefor...
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JP4202145B2 |
Methods, systems and apparatus for photo-processing of fluids, particularly complex fluids, such as blood products, pharmaceuticals, injectables and vaccines, are provided. The disclosed methods and systems employ non-laser light source(...
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JP4197808B2 |
The method involves arranging a supply duct in a substrate (2), which opens out close to the tip (3) or edge to be formed at the surface of the substrate. The supply duct is used to supply a chemical near to the tip or edge and electroma...
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JP2008539796A |
The invention provides novel processes for manufacturing autologous T cells, transducing T cells and expanding the transduced T cell population.
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JP4171755B2 |
To provide a method and apparatus for the encapsulation of biologically-active substances in red blood cells, characterized by an optionally automated, continuous-flow, self-contained electroporation system. The blood is introduced into ...
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JP4163686B2 |
The present invention relates to a simple, efficient, and accelerated method for enzyme-catalyzed in vitro modification and synthesis of nucleic acid using uninterrupted and brief microwave irradiation at a frequency ranging between 2300...
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JP2008194055A |
To provide isolation of an inner cell mass (ICM) by using a laser ablation technique, safe and simple and commercially practicable without using an animal-originated antibody or animal-originated serum and without carrying out difficult ...
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JP2008163134A |
To provide a preparation method of an alternative fuel contributing to obtainment of a high quality biodiesel fuel in a simple process without use of a chemical.The preparation method of an alternative fuel for obtaining a biodiesel fuel...
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JP2008521437A |
Techniques for computerized electroporation. An electroporation apparatus may be controlled according to one of a plurality of previously-saved, user-defined processing protocols. A processing log associated with a processing protocol ma...
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JP4104599B2 |
A method and system for generating modulated optical vortices. Optical vortices can be used for a variety of applications, such as applying controlled torque or controlled force patterns to objects from a few nanometers to hundreds of mi...
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JP4099601B2 |
To obtain a culture system capable of culturing an aerobically chemically independent nutritive bacterium in an extremely high proliferation rate to a high concentration (in the case of a ferrooxidans bacterium, 1×10<10> cells/ mL). Thi...
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JP2008099706A |
To provide a method for isolation of inner cell mass (ICM) using laser ablation without carrying out troublesome immunological surgery, excluding use of animal-originated antibody or animal-originated blood serum, safety, simple and comm...
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JPWO2005123906A1 |
To provide a method for increasing insulin production and / or secretion at a cellular level in a simple and efficient manner. An insulin-producing cell is exposed to an electromagnetic field having a frequency of 50 Hz ± 1 Hz or 60 Hz ...
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JP4070611B2 |
A method for introducing biologically active molecules into animal or human cells using an electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution including HEPES and at least ...
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JP2008035834A |
To provide an accelerating method for culturing cells by which the differentiation functions of the cells are precisely and rapidly manifested, and to provide a cell-cultured product and an artificial internal organ obtained by the metho...
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JP2008502401A |
A method of treating skin and/or skin conditions or disorders including chronoaging by the application of ultrasound.
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JP4027796B2 |
The present invention concerns a process for producing proteins in which a stream of liquid medium comprising at least one yeast, bacterium or mammalian cell undergoes electropulsing, and the proteins that are liberated are recovered. Th...
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JP2007535943A |
A method and apparatus for electroporation includes placing a mixture of bacterial suspension and transforming DNA into an electroporation cuvette. The resulting sample is subjected through a current-limiting device to a complex 5 wavefo...
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JP2007532284A |
An apparatus and method for electromagnetic treatment of living tissues and cells comprising: configuring at least one waveform according to a mathematical model having at least one waveform parameter, said at least one waveform to be co...
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JP4007433B2 |
A living cell electropermeation device, has at least two electrodes, a non-fatiguing voltage or current is passed to the electrodes through a controllable power semiconductor for a time interval. The development of the potential or the c...
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JP3974751B2 |
Disclosed are methods using neutral red to mediate the interconversion of chemical and electrical energy. Electrically reduced neutral red has been found to promote cell growth and formation of reduced products by reversibly increasing t...
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JP2007523671A |
A portable device for applying therapeutic electrical signals and/or electromagnetic fields to a patient's knee for the treatment of osteoarthritis and other diseases, defects and injuries. The device is operable in several modes to deli...
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JP3959652B2 |
To improve the efficiency of the subject reaction such as clinical tests, food tests or environmental tests by reacting an RNA or a DNA with a nucleic acid having a sequence complementary thereto while applying ultrasonic waves with a mo...
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JP2007521495A |
To separate an analyte in a solution, fixed to magnetic particles, the particles form a sediment to be separated in a number of bodies. At least one body (22) of magnetic particles is formed in an initial vessel (10) in a base body (2), ...
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JP2007514407A |
The invention relates to methods and apparatuses for introducing and releasing substances into and out of cells, and more specifically to methods and apparatuses for transiently permeabilizing a living cell so that any one or more of a v...
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JP2006526575T6 |
The improved method of the bioavailability of a biologically active substance includes applying the biologically active substance to laser radiation. Laser radiation changes a biologically active substance and the reaction about the subs...
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