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Title:
マイクロRNA(miRNA)などの調節性RNAの機能分析で使用するための核酸及びライブラリー
Document Type and Number:
Japanese Patent JP2010523100
Kind Code:
A
Abstract:
The invention relates to a nucleic acid comprising the following contiguous elements arranged in the 5 prime to 3 prime direction; a promoter; a selectable marker; a cloning site for receipt of a nucleic acid segment, said segment comprising a candidate miRNA target sequence; and a poly adenylation signal, said elements arranged such that a transcript directed by said promoter comprises said selectable marker, said candidate miRNA target sequence, and said poly adenylation signal in that order. Suitably the miRNA test sequence is or is derived from a 3′UTR. The invention also relates to methods for making and screening libraries.

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Inventors:
Gayken Johannes Adrinus
Mohamed Azim
Application Number:
JP2010501586A
Publication Date:
July 15, 2010
Filing Date:
April 04, 2008
Export Citation:
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Assignee:
KINGS COLLEGE LONDON
International Classes:
C12N15/09; C12N15/113; C12Q1/68
Domestic Patent References:
JPWO2005063980A12007-12-20
JPH06504432A1994-05-26
JP2003513619A2003-04-15
Foreign References:
WO2007034977A12007-03-29
WO2001053481A12001-07-26
Other References:
JPN6013020774; ｎａｔｕｒｅ　ｇｅｎｅｔｉｃｓ Ｖｏｌ．３６，　Ｎｏ．２, 2004, ｐｐ．１９０-１９６
Attorney, Agent or Firm:
Masaki Hirota



 
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