アグリンのヒトC44断片に特異的に結合することができるモノクローナル抗体を産生するハイブリドーマ細胞系の製造方法

Title:

アグリンのヒトC44断片に特異的に結合することができるモノクローナル抗体を産生するハイブリドーマ細胞系の製造方法

Document Type and Number:

Japanese Patent JP2014507942

Kind Code:

A

Abstract:

A method for the production of a hybridoma cell lines producing monoclonal antibodies capable to specifically binding to a human C44-fragment of agrin, comprising administering to wild-type-mice an immunizing amount of C44y¥4-fragment of agrin, isolating antibody producing cells from the immunized mice, fusing them with a myeloma cell line, growing the fused cells in a selection medium, screening the antibodies in the supernatants of hybridoma cells for binding to C44-fragment of agrin and isolating the hybridoma cells producing the desired monoclonal antibodies

Inventors:

Dahinden, Pius
Hetvar, Stephan
Fly blead, yan willem

Application Number:

JP2013552126A

Publication Date:

April 03, 2014

Filing Date:

January 13, 2012

Export Citation:

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Assignee:

Neurotune Actien Gezel Shaft

International Classes:

C12N5/10; A61K39/395; A61P11/00; A61P13/12; A61P25/00; C07K16/18; C12N15/02; C12N15/09; C12P21/08; G01N33/53

Domestic Patent References:

JP2010526994A

2010-08-05

Foreign References:

WO2011026615A1	2011-03-10
WO2008074813A2	2008-06-26

Other References:

JPN6014053705; ＨＯＣＨ，Ｗ．　ｅｔ　ａｌ．: 'Ｄｅｖｅｌｏｐｍｅｎｔａｌ　ｒｅｇｕｌａｔｉｏｎ　ｏｆ　ｈｉｇｈｌｙ　ａｃｔｉｖｅ　ａｌｔｅｒｎａｔｉｖｅｌｙ　ｓｐｌｉｃｅｄ　ｆｏｒｍｓ　ｏｆ　ａｇｒｉｎ．' ＮｅｕｒｏｎＶｏｌ．１１，　Ｎｏ．３, 199309, ｐｐ．４７９-９０
JPN6014053703; ＨＯＣＨ，Ｗ．　ｅｔ　ａｌ．: 'Ｓｔｒｕｃｔｕｒａｌ　ｄｏｍａｉｎｓ　ｏｆ　ａｇｒｉｎ　ｒｅｑｕｉｒｅｄ　ｆｏｒ　ｃｌｕｓｔｅｒｉｎｇ　ｏｆ　ｎｉｃｏｔｉｎｉｃ　ａｃｅｔｙｌｃｈｏｌｉｎｅ　ｒｅｃｅｐｔｏｒｓ．' ＥＭＢＯ　Ｊ．Ｖｏｌ．１３，　Ｎｏ．１２, 19940615, ｐｐ．２８１４-２１

Attorney, Agent or Firm:

Shoichi Okuyama
Arihara Koichi
Matsushima Tetsuo
Hidefumi Kawamura
Ayako Nakamura
Satoshi Morimoto
Kyoko Tsunoda
Yu Tanaka
Tokumoto Koichi
Atsushi Watanabe
Mai Kodama
Akiko Mizushima
Toru Masuya

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