Title:
【発明の名称】蛍光共鳴エネルギー移動を利用する核酸分析方法
Document Type and Number:
Japanese Patent JP3439221
Kind Code:
B2
Abstract:
A method for detecting the presence of a target nucleotide or sequence of nucleotides in a nucleic acid is disclosed. The method is comprised of forming an oligonucleotide labeled with two fluorophores on the nucleic acid target site. The doubly labeled oligonucleotide is formed by addition of a singly labeled dideoxynucleoside triphosphate to a singly labeled polynucleotide or by ligation of two singly labeled polynucleotides. Detection of fluorescence resonance energy transfer upon denaturation indicates the presence of the target. Kits are also provided. The method is particularly applicable to genotyping.
Inventors:
Quoc, Pui Yang
Chen, Chang Ning
Chen, Chang Ning
Application Number:
JP52301397A
Publication Date:
August 25, 2003
Filing Date:
December 17, 1996
Export Citation:
Assignee:
Washington University
International Classes:
C12Q1/68; C12Q1/6818; C12N15/09; (IPC1-7): C12N15/09; C12Q1/68
Other References:
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ，１９８８年 Ｖｏｌ．８５，ｐ．８７９０−８７９４
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ，１９９５年 ５月，Ｖｏｌ．９２，ｐ．４３４７−４３５１
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ，１９９５年 ５月，Ｖｏｌ．９２，ｐ．４３４７−４３５１
Attorney, Agent or Firm:
Yoshio Kawaguchi (4 outside)