Title:
哺乳類遺伝子発現のためのヒトハイブリッド宿主細胞
Document Type and Number:
Japanese Patent JP4332300
Kind Code:
B2
Abstract:
Human/human hybrid cells were made via fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8). The fusion cells are useful as host cells for the recombinant expression of mammalian genes. The advantages of using these hybrid clones of human kidney- and B-cells, called HKBs, for mammalian gene expression, include (i) the cells are negative for immunoglobulin expression, (ii) the cells grow easily in plasma protein-free medium (with or without the addition of recombinant insulin) as suspension cultures in a shake flask or in a fermenter (iii) the cells are very susceptible for transfection of DNA, and (iv) the cells secrete high levels of heterologous recombinant proteins, such as recombinant monoclonal antibodies, soluble ICAM-1, rIL-4, and rFVIII.
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Inventors:
Chiyo, Miyun-Sam
Application Number:
JP2000586896A
Publication Date:
September 16, 2009
Filing Date:
December 08, 1999
Export Citation:
Assignee:
BAYER CORPORATION
International Classes:
C07K2/00; C12N5/10; C12N5/02; C12N5/07; C12N5/073; C12N5/16; C12N5/22; C12N5/24; C12N15/01; C12N15/02; C12N15/07; C12N15/08; C12N
Domestic Patent References:
| JP2002531137A | ||||
| JP57040411A |
Other References:
J. Biomed. Sci.,2002年,vol. 9,p. 631-638
Gene,1989年,vol. 81,p. 139-149
Mol. Cell Biol.,1985年,vol. 5, no. 8,p. 2051-2060
Proc. Natl. Acad. Sci.,1983年,vol. 80,p. 2762-2766
Gene,1989年,vol. 81,p. 139-149
Mol. Cell Biol.,1985年,vol. 5, no. 8,p. 2051-2060
Proc. Natl. Acad. Sci.,1983年,vol. 80,p. 2762-2766
Attorney, Agent or Firm:
Patent business corporation Odashima patent office