Title:
高マンノースタンパク質および高マンノースタンパク質の製造方法
Document Type and Number:
Japanese Patent JP5008245
Kind Code:
B2
Abstract:
The invention features a method of producing a high mannose glucocerebrosidase (hmGCB) which includes: providing a cell which is capable of expressing glucocerebrosidase (GCB), and allowing production of GCB having a precursor oligosaccharide under conditions which prevent the removal of at least one mannose residue distal to the pentasaccharide core of the precursor oligosaccharide of GCB, to thereby produce an hmGCB preparation. Preferably, the condition which prevents the removal of at least one mannose residue distal to the pentasaccharide core is inhibition of a class 1 processing mannosidase and/or a class 2 processing manosidase. The invention also features an hmGCB preparation and methods of using an hmGCB preparation.
Inventors:
Kinoshita, Carroll A
Plush Santo, Mishra
Borrowski, Marian
Francis-Daniel, Peter
Plush Santo, Mishra
Borrowski, Marian
Francis-Daniel, Peter
Application Number:
JP2002520848A
Publication Date:
August 22, 2012
Filing Date:
August 17, 2001
Export Citation:
Assignee:
Shire Human Genetic Therapy Inc.
International Classes:
C12N9/24; A61K38/43; C12N15/09; A61K38/47; A61P3/06; C12N5/10; C12N9/99; C12N15/113; C12N15/56; C12P21/00; C12P21/02
Domestic Patent References:
| JP3503721A |
Other References:
DNA,1988年,vol.7 no.2,pp.99-106
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,1986年,vol.141 no.2,pp.452-458
THE JOURNAL OF BIOLOGICAL CHEMISTRY,1990年,vol.265 no.26,pp.15599-15605
Biochimica et Biophysica Acta,1981年,vol.673,pp.425-434
Proc. Natl. Acad. Sci. USA,1990年,vol.87,pp.1913-1916
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,1986年,vol.141 no.2,pp.452-458
THE JOURNAL OF BIOLOGICAL CHEMISTRY,1990年,vol.265 no.26,pp.15599-15605
Biochimica et Biophysica Acta,1981年,vol.673,pp.425-434
Proc. Natl. Acad. Sci. USA,1990年,vol.87,pp.1913-1916
Attorney, Agent or Firm:
Yanagita Seiji
Go Sakuma
Go Sakuma