Title:
３ＨＨ単位含有共重合ＰＨＡを生産する形質転換体、及び当該ＰＨＡの製造方法
Document Type and Number:
Japanese Patent JP7001596
Kind Code:
B2
Abstract:
Provided are a transformant that produces a copolymerized PHA containing 3HH units in a higher composition proportion; and a method for producing a copolymerized PHA, using this transfonnant. The transformant is a transformant that produces a copolymerized PHA containing 3HH units, in which a gene encoding an enzyme having trans-2-enoyl-CoA hydratase activity and (R)-3-hydroxyacyl-CoA dehydrogenase activity is introduced into a prokaryotic microorganism having a PHA synthetase gene capable of synthesizing the copolymerized PHA containing the 3HH units. The method is a method for producing a copolymerized PHA containing 3HH units, which includes a step of culturing this transformant.
Inventors:
Naoshi Arikawa
Keiji Matsumoto
Keiji Matsumoto
Application Number:
JP2018529766A
Publication Date:
February 03, 2022
Filing Date:
July 13, 2017
Export Citation:
Assignee:
Kaneka Corporation
International Classes:
C12N1/21; C12P7/62
Foreign References:
| WO2001009364A1 |
Other References:
HADDOUCHE Ramdane et al.,Engineering polyhydroxyalkanoate content and monomer composition in the oleaginous yeast Yarrowia li,Appl. Microbiol. Biotechnol.,2011年,Vol.91, No.5,pp.1327-1340
SATO Shunsuke et al.,Regulation of 3-hydroxyhexanoate composition in PHBH synthesized by recombinant Cupriavidus necator,Journal of Bioscience and Bioengineering,2015年,Vol.120, No.3,pp.246-251
INSOMPHUN Chayatip et al.,Improved artificial pathway for biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) with,Metabolic Engineering,2015年,Vol.27,pp.38-45
HAATAJA Tatu J. K. et al.,Peroxisomal multifunctional enzyme type 2 from the fruitfly: dehydrogenase and hydratase act as sepa,Biochem. J.,2011年,Vol.435, No.3,pp. 771-781
SATO Shunsuke et al.,Regulation of 3-hydroxyhexanoate composition in PHBH synthesized by recombinant Cupriavidus necator,Journal of Bioscience and Bioengineering,2015年,Vol.120, No.3,pp.246-251
INSOMPHUN Chayatip et al.,Improved artificial pathway for biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) with,Metabolic Engineering,2015年,Vol.27,pp.38-45
HAATAJA Tatu J. K. et al.,Peroxisomal multifunctional enzyme type 2 from the fruitfly: dehydrogenase and hydratase act as sepa,Biochem. J.,2011年,Vol.435, No.3,pp. 771-781
Attorney, Agent or Firm:
Aiko Patent Office, a patent business corporation