Title:
ALKALI PECTATE LYASE
Document Type and Number:
Japanese Patent JP3913898
Kind Code:
B2
Abstract:
PROBLEM TO BE SOLVED: To obtain a novel alkali pectase lyase that manifest excellent pectase lyase activity under high alkali conditions, can be produced in an industrial scale and is useful as an enzyme for detergent formulations, fiber treatment enzymes and the like.
SOLUTION: This enzyme is obtained by culturing a micro-organism in Bacillus, e.g. Bacillus sp. KSM-P539 (FERM P-15991) and has the following enzymologic properties: the action: it cleaves the α-1,4-bond in a pectin (e.g. polygalact-uronic acid) through the β-elimination reaction and gives a double bond to the C4-C5 position in the non-reducible terminal to produce an unsaturated oligogalacturonide; the substrate specificity: it acts on (proto)pectin and (acid-soluble) pectic acid; the optimal reaction: the enzymatic reaction is optimally carried out at 30°C at 11 pH (in a 50 mM glycine-sodium hydroxide buffer solution) in the substrate of pectic acid; the stable pH: 4-8; the optimal reaction temperature: at about 45°C; heat resistance: resistant to about 40°C; the molecular weight: about 16 kDa; the isoelectric point: near 10 pH.
Inventors:
Norihiko Higaki
Yoshimatsu Tadashi
Kenzo Koike
Atsushi Rinmatsu
Yoko Kaito
Toru Kobayashi
Decorosa Deal Sterio
Sylvan Gerard El Savio
Yoshimatsu Tadashi
Kenzo Koike
Atsushi Rinmatsu
Yoko Kaito
Toru Kobayashi
Decorosa Deal Sterio
Sylvan Gerard El Savio
Application Number:
JP12738598A
Publication Date:
May 09, 2007
Filing Date:
May 11, 1998
Export Citation:
Assignee:
Kao Corporation
International Classes:
C12N9/88; C11D3/386; C12N1/20; C12R1/07; (IPC1-7): C12N9/88; C12N1/20; //C11D3/386; (C12N9/88; C12R1:07); (C12N1/20; C12R1:07)
Other References:
Arch. Biochem. Biophy.,1995年,Vol. 323(2),p. 352-360
Arch. Biochem. Biophy.,1987年,Vol. 258(1),p. 196-205
Can. J. Microbiol.,1980年,Vol. 26(3),p. 377-384
Appl. Environ. Microbiol.,1995年,Vol. 61(10),p. 3580-3585
J. Bacteriol.,1991年,Vol. 173(5),p. 1765-1769
J. Bacteriol.,1986年,Vol. 167(1),p. 117-123
J. Bacteriol.,1987年,Vol. 169(6),p. 2774-2780
Arch. Biochem. Biophy.,1987年,Vol. 258(1),p. 196-205
Can. J. Microbiol.,1980年,Vol. 26(3),p. 377-384
Appl. Environ. Microbiol.,1995年,Vol. 61(10),p. 3580-3585
J. Bacteriol.,1991年,Vol. 173(5),p. 1765-1769
J. Bacteriol.,1986年,Vol. 167(1),p. 117-123
J. Bacteriol.,1987年,Vol. 169(6),p. 2774-2780
Attorney, Agent or Firm:
Alga Patent Office, a patent business corporation
Miyuki Ariga
Toshio Takano
Toshio Nakajima
Hiromi Matoba
Miyuki Ariga
Toshio Takano
Toshio Nakajima
Hiromi Matoba
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