Title:
BIOTINYL REAGENT AND BIOTINYLATION USING SAID REAGENT
Document Type and Number:
Japanese Patent JPS63246382
Kind Code:
A
Abstract:
NEW MATERIAL:A compound shown by formula I (X is -COO-, -CONH2 or H; n is 1W10).
EXAMPLE: A salt of N-d-biotinyl-3-nitro-2-pyridylsulfenyl-L-cysteine N.
USE: A biotinyl reagent useful for avidin-biotin-N-system and important in the filed of purification of preformed chemical mediator as well as immune analysis, in vivo microanalysis and chemical analysis.
PREPARATION: A compound shown by formula II is reacted with d-biotinyl-N hydroxysuccinimide ester. The starting raw material shown by the formula II is obtained by treating N-t-butoxycarbonyl-S-3-nitro-2-pyridylphenylcysteine shown by formula III with an acid.
Inventors:
HAYASHI YOSHIO
EZAWA KUNIO
EZAWA KUNIO
Application Number:
JP7960087A
Publication Date:
October 13, 1988
Filing Date:
April 02, 1987
Export Citation:
Assignee:
CALPIS FOOD IND CO LTD
International Classes:
G01N33/68; C07D495/04; (IPC1-7): C07D495/04; G01N33/68
Attorney, Agent or Firm:
Toda Chikio