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Title:
DIRECT RESCUE OF RECOMBINANT VIRUS FROM CELL DNA OF TISSUE, AND METHOD FOR AMPLIFICATION
Document Type and Number:
Japanese Patent JP3943048
Kind Code:
B2
Abstract:

PROBLEM TO BE SOLVED: To provide a method for isolating an AAV virus from a cell DNA of an NHP tissue, and to provide a kit useful for carrying out the method.
SOLUTION: The cell DNA of a nonhuman primate (NHP) is transfected to 293 cells, and the virus is rescued. The rescued virus is amplified by serial passagees in the presence of an adenovirus helper function.


Inventors:
Gunpin Gao
The Ames M. Wilson
Mauricio Earl Albira
Application Number:
JP2003116878A
Publication Date:
July 11, 2007
Filing Date:
April 22, 2003
Export Citation:
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Assignee:
The Trustees of the University of Pennsylvania
International Classes:
C12N7/02; C12N15/09; C12N15/35; C12N15/861; C12N15/864; C12P19/34; C12Q1/70; (IPC1-7): C12N7/02; C12N15/09; C12Q1/70
Attorney, Agent or Firm:
Heikichi Odashima