To provide a DNA probe chip which improves a hybridization rate on the surface of a solid chip, carries out a measurement in a short period of time with high sensitivity and causes less pseudopositive hybridizations, and also to provide a method of producing the DNA probe chip and a hybridization method for the DNA probe chip.

The surface of a probe fixing region in the DNA probe chip is made irregular, thereby improving the fixation amount of probe without increasing probe densities.