DNA ENCODING MODIFIED SIGNAL PEPTIDE

Japanese Patent JP3136356

B2

PROBLEM TO BE SOLVED: To obtain the subject new DNA encoding a recombinant protein having a sequence of adding a basic amino acid to the N-terminal region of a wild type chicken lysozyme signal peptide having a specific amino acid sequence and capable of being produced in a host cell such as of a yeast, in a large amount.
SOLUTION: This DNA is a new DNA encoding a modified signal peptide consisting of an amino acid sequence obtained by adding at least one basic amino acid to the N-terminal region of the amino acid sequence of a wild type chicken lysozyme signal peptide, can widely increase the amount of a recombinant type protein in a host cell such as of a yeast, and can realize a cost down, or the like, of various recombinant type protein preparations such as a cytokine. The DNA is obtained by preparing a primer used for modifying the gene based on the amino acid sequence of the objective modified signal peptide by a phosphate diester method, or the like, and amplifying the DNA encoding the wild type chicken lysozyme signal peptide by a PCR method by using the obtained primer.

Yoshinori Tsuchiya
Junsuke Shirai

JP35606298A

February 19, 2001

December 15, 1998

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Director, Animal Health Testing Station, Ministry of Agriculture, Forestry and Fisheries
Yoshinori Tsuchiya

C12N15/09; C07K14/465; C07K14/52; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N15/00; C12P21/02; C12R1/865; C12R1/91; (IPC1-7): C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10

JP3183479A

Ｂｉｏｃｈｅｍ．Ｃｅｌｌ Ｂｉｏｌ．，７１，ｐ４０１−４０５，１９９３
Ｊ．Ｂｉｏｌ．Ｃｈｅｍ．，２６５（８），ｐ．４３５８−４３６３，１９９０

Yusuke Hiraki (1 person outside)

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