Title:
マイコプラズマを検出する方法
Document Type and Number:
Japanese Patent JP6704565
Kind Code:
B2
Abstract:
An object of the present invention is to provide a detection method for Mycoplasma by which a greater number of Mycoplasma species can be more quickly and easily detected with high sensitivity and accuracy, a set of a forward primer, a reverse primer and a probe for the detection and a kit containing the set. Mycoplasma in a test sample is detected by a multiplex real time quantitative PCR using one or more forward primers, each of which is an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 30 nucleotides in the nucleotide sequence represented by SEQ ID No: 1, and which contains a nucleotide sequence at nucleotide positions 14 to 24 in SEQ ID No: 1; reverse primers, each of which is an oligonucleotide consisting of a nucleotide sequence, which is selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in the one or more nucleotide sequences represented by SEQ ID Nos: 14 and 17 to 20; and a probe(s), which is an oligonucleotide, which consists of a nucleotide sequence selected from the group consisting of nucleotide sequences each consisting of continuous 17 to 26 nucleotides in the nucleotide sequence represented by SEQ ID No: 33, or which consists of a complementary nucleotide sequence thereto.
Inventors:
Norio Shimizu
Hideyuki Takahashi
Hideyuki Takahashi
Application Number:
JP2016547698A
Publication Date:
June 03, 2020
Filing Date:
September 07, 2015
Export Citation:
Assignee:
Tokyo Medical And Dental University, Medical Hospital
Gc Rinfotech Co., Ltd.
Gc Rinfotech Co., Ltd.
International Classes:
C12Q1/689; C12M1/00; C12M1/34; C12N15/11; C12Q1/6837; C12Q1/6844
Domestic Patent References:
| JP4004899A | ||||
| JP6098800A | ||||
| JP5000088A |
Foreign References:
| WO2005078102A1 | ||||
| KR1020090081039A |
Other References:
STAKENBORG, T., et al.,Vet. Res. Commun.,2006年,vol.30, issue 3,p.239-247
FRAGA, AP., et al.,Braz. J. Microbiol.,2013年,vol.44, no.2,p.505-510
高橋秀行ほか,マルチプレックスqPCR法による新規マイコプラズマ検査法の開発,再生医療,2014年 1月,vol.13, 増刊号,p.357(P-2-190)
FRAGA, AP., et al.,Braz. J. Microbiol.,2013年,vol.44, no.2,p.505-510
高橋秀行ほか,マルチプレックスqPCR法による新規マイコプラズマ検査法の開発,再生医療,2014年 1月,vol.13, 増刊号,p.357(P-2-190)
Attorney, Agent or Firm:
Masaki Hirota
Seiji Ozawa
Yusaku Tokai
Kazuhiro Matsuda
Makoto Horiuchi
Masako Yamauchi
Shuichi Sonomoto
Akihiro Yamamura
Satoshi Morikawa
Hiroyuki Tomita
Seiji Ozawa
Yusaku Tokai
Kazuhiro Matsuda
Makoto Horiuchi
Masako Yamauchi
Shuichi Sonomoto
Akihiro Yamamura
Satoshi Morikawa
Hiroyuki Tomita