Title:
【発明の名称】ストレプトキナーゼをコードする遺伝子の単離および発現方法、得られるヌクレオチド配列、組換体DNAおよび形質転換微生物
Document Type and Number:
Japanese Patent JP3127298
Kind Code:
B2
Abstract:
The present invention relates to the field of biotechnology and genetic engineering and in particular a novel nucleotide sequence which codes for a streptokinase, as well as the recombinant DNA obtained therefrom which is used for the transformation of various host organisms. The technical object which is pursued with the proposed solution is to produce the protein in commercially useful quantities, by the recombinant method, with the possibility of alternatively using different hosts to obtain a high-quality streptokinase capable of being used as a thrombolytic agent in the treatment of different disorders. The present invention is based on the isolation of a new gene which codes for streptokinase from Streptococcus equisimilis of type C (strain ATCC-9542) and the cloning and expression thereof in prokaryotic (E. coli) and eukaryotic (Pichia pastoris) hosts, for which it includes the vehicles of expression which contain the genetic sequences of said gene, as well as the microorganisms transformed with these vectors capable of producing streptokinase. The protein obtained thereby can be used in clinical medicine as a therapeutic agent, in the treatment of disorders such as thromboembolic obstructions including coronary thrombosis.
Inventors:
Mario Pablo Estrada Garcia
Aimee Perez Felipe
Roger Rubiela Chaplain
Ricardo Seriano Doce
Luciano Francisco Hernandez Marjero
Pedro Rodriguez Corrasso
Anai Cell Castro Rameeless
Emilio Ambre Munoz Munoz
Walfried Bravo Martinez
Magallas Campos Somavilla
Alicita Pedrasa Fernandez
Jose de Jesus de la Fuente Garcia
Luis Saturnino Elera Martinez
Aimee Perez Felipe
Roger Rubiela Chaplain
Ricardo Seriano Doce
Luciano Francisco Hernandez Marjero
Pedro Rodriguez Corrasso
Anai Cell Castro Rameeless
Emilio Ambre Munoz Munoz
Walfried Bravo Martinez
Magallas Campos Somavilla
Alicita Pedrasa Fernandez
Jose de Jesus de la Fuente Garcia
Luis Saturnino Elera Martinez
Application Number:
JP20160090A
Publication Date:
January 22, 2001
Filing Date:
July 31, 1990
Export Citation:
Assignee:
Centro de Heni Area Henetica Ibio Technolohia
International Classes:
A61K38/46; A61P7/02; C07K14/315; C12N1/19; C12N1/21; C12N9/70; C12N15/52; C12N15/58; C12N15/81; C12N15/09; A61K38/00; C12R1/19; C12R1/465; C12R1/84; (IPC1-7): C12N15/09; A61K38/46; A61P7/02; C12N1/19; C12N1/21; C12N9/70
Domestic Patent References:
| JP62296881A |
Other References:
Ｐｒｏｃ．Ｎａｔｌ．Ａｃａｄ．Ｓｃｉ．ＵＳＡ，８１（１９８４），ｐ．３５５７−３５６１
Attorney, Agent or Firm:
Masashi Yoshioka (1 person outside)