To provide an improved system for nucleic acid cloning.
The present invention provides the improved system for linking nucleic acids to one another. In particular, the present invention provides techniques for producing DNA product molecules that may be easily and directly ligated to recipient molecules. The invention further provides methods for directed ligation of product molecules (i.e., for selective ligation of certain molecules within a collection of molecules), and also for methods of exon shuffling, in which multiple different product molecules are produced in a single ligation reaction. The inventive DNA manipulation system is readily integrated with other nucleic acid manipulation systems, such as ribozyme-mediated systems, and also is susceptible to automation.
COLJEE VINCENT W
DONAHUE WILLIAM
MIKHEEVA SVETLANA
Takaaki Yasumura
Natsuki Morishita