Title:
IN VIVO ASSESSMENT METHOD OF MOLECULAR TARGET SUBSTANCE BINDING TO HUMAN SPECIFIC MOLECULE
Document Type and Number:
Japanese Patent JP2015152395
Kind Code:
A
Abstract:
PROBLEM TO BE SOLVED: To provide a method capable of easily and swiftly producing a transgenic non-human animal useful for assessing characteristics, pharmacological actions and safety of a molecular target substance or a target drug to a human specific molecule, and assessing the characteristics, pharmacological actions and safety of a molecular target substance or a target drug to a human specific molecule using the transgenic non-human animal.SOLUTION: In a BAC clone of a non-human animal, a gene encoding the human specific molecule is replaced by a homologue gene of a non-human animal of the gene encoding the human specific molecule to provide a recombinant BAC clone. The recombinant BAC clone is introduced as an expression vector in the non-human animal by a microinjection method to produce a transgenic non-human animal expressing the human specific molecule. The method for assessing characteristics of a test article, includes: administering the test article to the transgenic non-human animal; and assessing the characteristics of the test article within the organism of the transgenic non-human animal.
Inventors:
SHIODA AKIRA
UEDA MASAJI
ITO YUKIO
UEDA MASAJI
ITO YUKIO
Application Number:
JP2014025653A
Publication Date:
August 24, 2015
Filing Date:
February 13, 2014
Export Citation:
Assignee:
INST OF IMMUNOLOGY CO LTD
International Classes:
G01N33/50; A01K67/027; C07K16/18; C12N15/09; C12Q1/68; G01N33/15
Domestic Patent References:
| JP2012024019A | 2012-02-09 | |||
| JP2013543379A | 2013-12-05 | |||
| JP2009055911A | 2009-03-19 | |||
| JP2005503809A | 2005-02-10 |
Foreign References:
| WO2013064443A1 | 2013-05-10 | |||
| WO2012141798A1 | 2012-10-18 |
Attorney, Agent or Firm:
Yusuke Hiraki
Satoshi Fujita
Nobuto Oki
Satoshi Fujita
Nobuto Oki