LENGTH DETERMINATION OF NUCLEIC ACID REPEAT SEQUENCES BY DISCONTINUOUS PRIMER EXTENSION

Title:

LENGTH DETERMINATION OF NUCLEIC ACID REPEAT SEQUENCES BY DISCONTINUOUS PRIMER EXTENSION

Document Type and Number:

Japanese Patent JP2010213709

Kind Code:

A

Abstract:

To provide a precise and reproducible method for determining the number of repeat units making up a repeat region of a nucleic acid repeat sequence.

Disclosed is a method for determining the number of repeat units in a repeat region of a target nucleic acid. The method includes discontinuous primer reactions, wherein the primers are extended in an individual increase corresponding to a single repeating unit. Following each increment of discrete primer extension, a detection step is performed in which a modulation in a signal is detected when the primer has been extended by an amount equal to the total length of a repeat region. Thus, by counting the number of increments of discrete primer extension required to cause a modulation in the signal, the number of repeat units making up the repeat region is determined.

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Inventors:

LIVAK KENNETH J
LOWE ADAM L
BLASBAND ANDREW J

Application Number:

JP2010092670A

Publication Date:

September 30, 2010

Filing Date:

April 13, 2010

Export Citation:

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Assignee:

APPLIED BIOSYSTEMS LLC

International Classes:

C12N15/09; C12Q1/6827; C12Q1/68; C12Q1/6837; C12Q1/6858; C12Q1/6874; G01N21/78; G01N33/53; G01N33/566; G01N37/00

Attorney, Agent or Firm:

Hidesaku Yamamoto
Takaaki Yasumura
Natsuki Morishita

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