Title:
METHOD AND COMPOSITION FOR DETERMINING PURITY OF CHEMICALLY SYNTHESIZED NUCLEIC ACID
Document Type and Number:
Japanese Patent JP2012107019
Kind Code:
A
Abstract:
To provide a method for separating synthetic oligonucleotides protected from fully deprotected synthetic oligonucleotides.
A method for separating the synthetic oligonucleotides protected from fully deprotected synthetic oligonucleotides includes: a step of bringing a mixture of protected synthetic oligonucleotides and fully deprotected synthetic oligonucleotides into contact with antibodies that specifically binds to the synthesized oligonucleotides covalently binding to an organic protecting group but not binding thereto if the organic protecting group does not covalently bind; and a step of purifying the synthesized oligonucleotides fully deprotected from the protected oligonucleotides to which the antibodies bind.
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Inventors:
AGRIS PAUL F
MITCHELL LLOYD G
PEARCE CHRISTOPHER D J
MITCHELL LLOYD G
PEARCE CHRISTOPHER D J
Application Number:
JP2011286484A
Publication Date:
June 07, 2012
Filing Date:
December 27, 2011
Export Citation:
Assignee:
UNIV NORTH CAROLINA STATE
INTRONN
INTRONN
International Classes:
C07H21/02; G01N33/53; B01J20/281; C07H21/04; C07K16/18; C07K16/44; C12M1/00; C12N5/10; C12N15/09; C12P21/08; C12Q1/68; G01N30/88; G01N37/00
Domestic Patent References:
| JPH1180185A | 1999-03-26 |
Foreign References:
| US5221736A | 1993-06-22 |
Other References:
JPN6010031216; Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ Ｈｏｐｐｅ-Ｓｅｙｌｅｒ Ｖｏｌ．３７２, 1991, ｐ．８４５-８４８
JPN6010031216; Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ Ｈｏｐｐｅ-Ｓｅｙｌｅｒ Ｖｏｌ．３７２, 1991, ｐ．８４５-８４８
JPN6010031216; Ｂｉｏｌｏｇｉｃａｌ Ｃｈｅｍｉｓｔｒｙ Ｈｏｐｐｅ-Ｓｅｙｌｅｒ Ｖｏｌ．３７２, 1991, ｐ．８４５-８４８
Attorney, Agent or Firm:
Shoichi Okuyama
Arihara Koichi
Matsushima Tetsuo
Arihara Koichi
Matsushima Tetsuo