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Title:
METHOD FOR DESIGNING PRIMER FOR SELEX METHOD, METHOD FOR PRODUCING PRIMER, METHOD FOR PRODUCING APTAMER, DEVICE FOR DESIGNING PRIMER, AND COMPUTER PROGRAM AND RECORDING MEDIUM FOR DESIGNING PRIMER
Document Type and Number:
Japanese Patent JP2010239873
Kind Code:
A
Abstract:

To provide a design method whereby a primer for the SELEX method can be efficiently designed.

The method for designing a primer is featured by performing following steps using a computer: a step of generating primer candidate sequences (S1); a step of selecting a primer candidate sequence by evaluating the primer candidate sequences on the basis of predetermined standards (S2); a step of generating random pools including a plurality of nucleic acid sequences including random sequences and primer candidate sequences on the basis of the selected primer candidate sequence (S3); a step of selecting a random pool by predicting the structure of each of nucleic acid sequences of the random pools and by evaluating the predicted structure on the basis of predetermined standards (S4); and a step of determining to adopt a primer candidate sequence used in the selected random pool as a primer sequence (S5).


Inventors:
AKITOMI MINORU
KATO SHINTARO
YOSHIDA YOSHIHITO
Application Number:
JP2009089607A
Publication Date:
October 28, 2010
Filing Date:
April 01, 2009
Export Citation:
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Assignee:
NEC SOFTWARE LTD
International Classes:
C12N15/09; G16B25/20; C12M1/00; C12N15/115; G16B15/00
Domestic Patent References:
JP2009017793A2009-01-29
JP2008102675A2008-05-01
JP2008118923A2008-05-29
JP2008283943A2008-11-27
Attorney, Agent or Firm:
Koichiro Tsujimaru
Yumi Nakayama
Reiko Yoshida
Isaji So



 
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