Title:
混合物から変異された核酸の富化方法
Document Type and Number:
Japanese Patent JP6553598
Kind Code:
B2
Abstract:
The detection of the presence of rare somatic mutations from a biological sample is often challenging due to the simultaneous presence of a vast excess of wild-type DNA. The present invention describes methods that would allow the enrichment of mutant DNA by depleting amplifiable wild-type DNA.
Inventors:
Nancy Shane Bruner
Amaru Gupta
Kevin Jansen
Amaru Gupta
Kevin Jansen
Application Number:
JP2016521352A
Publication Date:
July 31, 2019
Filing Date:
November 25, 2014
Export Citation:
Assignee:
F. HOFFMANN-LA ROCHE AKTIENGESELLSCHAFT
International Classes:
C12N15/10; C12Q1/6806; C12Q1/6827; C12Q1/6876
Foreign References:
| WO2002086169A1 | ||||
| US6120992 | ||||
| WO2001005800A1 | ||||
| US6031098 | ||||
| WO2007084380A1 |
Other References:
JACKSON, B.A. et al.,"A versatile mismatch recognition agent: specific cleavage of plasmid DNA at a single base mispair.",BIOCHEMISTRY,1999年 4月13日,Vol.38, No.15,pp.4655-4662,doi: 10.1021/bi990255t
Attorney, Agent or Firm:
Atsushi Aoki
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Kazumi Nakamura
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Ryotaro Takei
Kazumi Nakamura