METHOD OF FORMING OF MATSUTAKE FUNGUS-INFECTED PINE SEEDLING BY COCULTIVATING MATSUTAKE FUNGUS AND ASEPTICALLY GERMINATED PINE SEEDLING

Title:

METHOD OF FORMING OF MATSUTAKE FUNGUS-INFECTED PINE SEEDLING BY COCULTIVATING MATSUTAKE FUNGUS AND ASEPTICALLY GERMINATED PINE SEEDLING

Document Type and Number:

Japanese Patent JP3947143

Kind Code:

B2

Abstract:

PROBLEM TO BE SOLVED: To provide a method for forming a Matsutake (Tricholoma matsutake) fungus-infected seedling by cocultivating a Matsutake fungus strain and an aseptically germinated pine seedling.
SOLUTION: In this method for forming the Matsutake fungus-infected pine seedling by cocultivating a Matsutake fungus and the aseptically germinated pine seedling, a mycelium powdered material which is obtained by powdering a fruit body of the Matsutake fungus given by subjecting the fungus to liquid culture in a PDB (potato dextrose broth) culture medium is inoculated in an amount in a dry base of 0.01-0.02mg per 1ml of sterile water on the bottom of a sterilized culture vessel. Then, a K-liquid culture medium which is specifically formulated and controlled to have a pH of 5.5 to 5.6 is dividedly injected into mixed bed soil which is obtained by mixing perlite and sphagnum peat moss in a ratio of 80 to 1-2 and arranged on the inoculated mycelium powdered material. Further, the culture vessel is covered with a lid, after the aseptically germinated seedling which is obtained by aseptically geminating a pine seed and has a length of ≤ 3cm is laid on an infection culture medium containing the mixed bed soil and the K-liquid culture medium and embedded therein. Furthermore, the pine nersery seedling and a Matsutake fungus culture are cocultivated at 15-25°C for 24 hr in a lighting condition of 10-40,000lux.

Inventors:

Mu-Chan Park
Sun-Gab Sim
Woo-Jotion

Application Number:

JP2003314273A

Publication Date:

July 18, 2007

Filing Date:

September 05, 2003

Export Citation:

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Assignee:

Gyeongsangbuk-do

International Classes:

A01G1/04; A01G23/00; A01H4/00; A01H5/00; A01H17/00; C12N1/14; (IPC1-7): A01G1/04; C12N1/14

Domestic Patent References:

JP2001120061A
JP9201129A
JP5211819A
JP9023756A
JP52006632A
JP2001148944A

Other References:

J.R.Norris, et al.,Methods in Microbiology ,Academic Press,1991年,VOL.23,P.75-94

Attorney, Agent or Firm:

Yusuke Hiraki
Ishii Teiji
Satoshi Fujita

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