Title:
腺性下垂体又はその前駆組織の製造方法
Document Type and Number:
Japanese Patent JP6789814
Kind Code:
B2
Abstract:
The present invention provides a method for inducing adenohypophysis or precursor tissue thereof in vitro from human pluripotent stem cells. The method includes culturing an aggregate of human pluripotent stem cells in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signal pathway to obtain a human cell aggregate containing a hypothalamus neuroepithelial tissue and a surface ectoderm, and further culturing the obtained human cell aggregate containing the hypothalamus neuroepithelial tissue and the surface ectoderm in suspension in a medium containing a bone morphogenetic protein signal transduction pathway activating substance and a substance acting on the Shh signal pathway to induce formation of hypophysial placode and/or Rathke's pouch in the surface ectoderm, thus obtaining a human cell aggregate containing 1) hypothalamus neuroepithelial tissue, and 2) hypophysial placode and/or Rathke's pouch.
More Like This:
Inventors:
Yoshiki Sasai
Osone Chimbun
Hidetaka Suga
Osone Chimbun
Hidetaka Suga
Application Number:
JP2016536000A
Publication Date:
November 25, 2020
Filing Date:
July 24, 2015
Export Citation:
Assignee:
RIKEN
Sumitomo Chemical Co., Ltd.
Sumitomo Chemical Co., Ltd.
International Classes:
C12N5/071; C12N5/0735; C12N5/079
Domestic Patent References:
| JP2013128476A |
Foreign References:
| WO2013065763A1 | ||||
| WO2013166488A1 | ||||
| WO2015077648A1 |
Other References:
Cell Reports, 2013, Vol.5, pp.1387-1402
Developmental Biology, 2013, Vol.379, pp.208-220
現代医学, 2013, Vol.61, No.2, pp.191-199
実験医学, 2013, Vol.31, No.16, pp.2645-2652
ブレインサイエンス・レビュー, 2014.02, Vol.2014, pp.99-112
Dev. Biol., 2010, Vol.348, No.2, pp.199-209
Okajimas Folia Anat. Jpn., 2000, Vol.77, No.4, pp.109-118
J. Steroid Biochem., 1989, Vol.32, No.1B, pp.183-191
Developmental Biology, 2013, Vol.379, pp.208-220
現代医学, 2013, Vol.61, No.2, pp.191-199
実験医学, 2013, Vol.31, No.16, pp.2645-2652
ブレインサイエンス・レビュー, 2014.02, Vol.2014, pp.99-112
Dev. Biol., 2010, Vol.348, No.2, pp.199-209
Okajimas Folia Anat. Jpn., 2000, Vol.77, No.4, pp.109-118
J. Steroid Biochem., 1989, Vol.32, No.1B, pp.183-191
Attorney, Agent or Firm:
Takashima Ichi
Mitsunori Kamata
Mitsunori Kamata