Title:
METHOD FOR PRODUCING PEPTIDE
Document Type and Number:
Japanese Patent JP2011223896
Kind Code:
A
Abstract:
To provide an efficient method for producing a peptide in order to produce a peptide using a culture that is easily solubilized and has high enzyme activity per unit.
An AET expression strain obtained by replacing an N-terminal signal peptide of amino acid ester transpeptidase (AET) derived from Sphingobacterium multivorum with an N-terminal signal peptide of amino acid ester transpeptidase derived from Erwinia carotovora and an AET expression strain in which an N-terminal signal peptide is deleted at various parts are constructed and localized in a cytosol to give an AET culture having excellent enzyme activity. The culture is mixed with a carboxy component and an amine component to form a peptide.
Inventors:
ABE TAKUMI
HIRATSUKA NORIHIRO
TAKEUCHI SONOKO
SUZUKI SHUNICHI
YOKOZEKI KENZO
HIRATSUKA NORIHIRO
TAKEUCHI SONOKO
SUZUKI SHUNICHI
YOKOZEKI KENZO
Application Number:
JP2010094407A
Publication Date:
November 10, 2011
Filing Date:
April 15, 2010
Export Citation:
Assignee:
AJINOMOTO KK
International Classes:
C12P21/02; C12N15/09
Attorney, Agent or Firm:
Hiroaki Sakai
Tadashi Terasaki
Takuya Kashima
Tadashi Terasaki
Takuya Kashima