To provide a method of producing male or female sterile plants and a method of producing enantiomerically pure D-Phosphinothricin (D-PPT).
The method includes the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, regenerating the thus transformed material into a plant, and applying the non-phytotoxic substance to the plant up to the time of male or female gamete formation and/or maturation. Thus, the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the gametes non-functional, wherein D-PPT is used as the non-phytotoxic substance and D-amino acid oxidase is used as the enzyme.
MITCHELL GLYNN
HADFIELD STEPHEN THOMAS
THOMPSON PAUL ANTHONY
VINER RUSSELL
ZHAN YONG
JPH0853477A | 1996-02-27 |
WO2001005982A1 | 2001-01-25 |
Takashi Ishida
Fukumoto product
Tetsuji Koga
Yoichi Watanabe
Kazuhiro Nakamura
Ryotaro Takei