ヒストンＨ３－リジントリメチル化を除去することによって体細胞核移入（ＳＣＮＴ）効率を増加させるための方法および組成物

Japanese Patent JP7277494

B2

The present invention provides methods and compostions to improve the efficiency of somatic cell nuclear transfer (SCNT) and the consequent production of nuclear transfer ESC (ntESC) and transgenic cells and/or non-human animals. More specifically, the present invention relates to the discovery that trimethylation of Histone H3-Lysine 9 (H3K9me3) in reprogramming resistant regions (RRRs) in the nuclear genetic material of donor somatic cells prevents efficient somatic cell nuclear reprogramming or SCNT. The present invention provide methods and compositions to decrease H3K9me3 in methods to improve efficacy of SCNT by exogenous or overexpression of the demethylase Kdm4 family and/or inhibiting methylation of H3K9me3 by inhibiting the histone methyltransferases Suv39h1 and/or Suv39h2.

JP6707636	NT cell storage method and its banking system
WO/2002/045490	METHOD OF CLONING ANIMALS
WO/2002/029000	METHODS OF MINIMIZING IMMUNOLOGICAL REJECTION OF A NUCLEAR TRANSFER FETUS

Chang Yi
Shogo Matoba

JP2021023834A

May 19, 2023

February 18, 2021

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The Children's Medical Center Corporation

C12N15/873; A01K67/027; C12N5/071; C12N5/073; C12N5/075; C12N5/10

JP2007117081A

WO2010033920A2

Mol. Cell. Biol., (2013) vol.33, no.5, p.974-983
Methods, (2008) vol.45, p.101-114
Database GenBank [online], Accessin No. BC156878, ,11-Dec-2007 uploaded, Strausberg, T.L. et al., Definition: Synthetic construct Mus musculus clone IMAGE:100062716 , MGC:190939 jumonji domain containing 2D (Jmjd2d) mRNA, encodes complet e protein
Open Biosystems製品カタログ2007, フナコシ株式会社,

Hatsushi Shimizu
Masao Haruna
Hirotaka Yamaguchi
Shun Jinbe
Ryuichi Inoue
Toshimitsu Sato
Koichi Niimi
Tomohiko Kobayashi
Hideki Kodera
Masato Ozeki
Kazuya Kawamoto

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