Title:
METHODS TO INCREASE OR DECREASE BONE DENSITY
Document Type and Number:
Japanese Patent JP2010213708
Kind Code:
A
Abstract:
To provide new sclerostin-protein antagonists, methods for identifying new sclerostin antagonists, molecules that can depress expression of SOST gene, and methods for identifying such molecules.
The invention provides identifying methods for depressing expression of the SOST gene including (a) adding osteoblast inducing culture medium in an amount sufficient for cells to express the SOST gene to mesenchyme cell, (b) bringing the cells in contact with test factors, (c) detecting the depression in the expression of the SOST gene in the absence of the test factors on comparison with the expression of the SOST gene in the presence of the test factors.
Inventors:
KUNG SUTHERLAND MAY S
GEOGHEGAN JAMES C
YU CHANGPU
LATHAM JOHN
GEOGHEGAN JAMES C
YU CHANGPU
LATHAM JOHN
Application Number:
JP2010091409A
Publication Date:
September 30, 2010
Filing Date:
April 12, 2010
Export Citation:
Assignee:
CELLTECH R & D INC
International Classes:
C12Q1/68; G01N33/50; A61K31/557; A61K31/5575; A61K31/56; A61K31/573; A61K31/575; A61K31/7088; A61K31/7105; A61K38/00; A61K38/40; A61K39/395; A61K45/00; A61K48/00; A61P19/08; A61P19/10; C12N15/09; C12N15/113; C12Q1/02; C12Q1/04; G01N33/15
Foreign References:
| WO2000032773A1 | 2000-06-08 |
Other References:
JPN6009042890; ＶＡＮ，Ｂ．Ｒ． ｅｔ ａｌ: 'ＢＭＰ-ａｎｔａｇｏｎｉｓｔ ｓｃｌｅｒｏｓｔｉｎ ｉｓ ｅｘｐｒｅｓｓｅｄ ｉｎ ｍｉｎｅｒａｌｉｚｅｄ ｂｏｎｅ ａｎｄ ｂｌｏｃｋｓ ＢＭＰ-ｉｎｄｕｃｅｄ ｂｏｎｅ ｆｏｒｍａｔｉｏｎ ｉｎ' Ｊｏｕｒｎａｌ ｏｆ Ｂｏｎｅ ａｎｄ Ｍｉｎｅｒａｌ Ｒｅｓｅａｒｃｈ Ｖｏｌ．１６， Ｎｏ．Ｓｕｐｐｌ, 2001, ｐ．Ｓ１６３
JPN6012031052; 村松正實編， 分子細胞生物学辞典， 株式会社東京化学同人， ２００２年２月， ｐ．３０３
JPN6012031052; 村松正實編， 分子細胞生物学辞典， 株式会社東京化学同人， ２００２年２月， ｐ．３０３
Attorney, Agent or Firm:
Sadao Kumakura
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai
Nobuo Ogawa
Atsushi Hakoda
Kenji Asai